Murine Model of Wound Healing

Louise Dunn; Hamish C. G Prosser; Joanne T. M. Tan; Laura Z. Vanags; Martin K. C. Ng; Christina A. Bursill

doi:10.3791/50265

JoVE Journal
Medicine

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JoVE Journal Medicine

Murine Model of Wound Healing

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05:39 min

May 28, 2013

DOI: 10.3791/50265-v

Louise Dunn^1,2, Hamish C. G Prosser^1,2, Joanne T. M. Tan^1,2, Laura Z. Vanags^1,2, Martin K. C. Ng^1,2,3, Christina A. Bursill^1,2

¹The Heart Research Institute, ²Sydney Medical School,University of Sydney , ³Cardiology Department,Royal Prince Alfred Hospital

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

The overall goal of this procedure is to create a wound, which is splinted, to prevent contraction and promote epithelialization sole proliferation and angiogenesis. This is accomplished by first preparing the dorsum and creating two full thickness excisional wounds either side of the midline. Next, a splint is placed over the wound and sutured in place.

The wound is then measured and treatments are applied. Lastly, an occlusive dressing is placed over the wound and the mouse is allowed to recover. Ultimately, the cutaneous excisional wound healing model is used to assess wound, epithelialization and associated processes, including proliferation and angiogenesis.

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