Micron Scale Constrictions

Micron-scale constrictions are narrow channels or openings with dimensions on the order of micrometers, where limited space changes how fluids, particles, or cells move and makes transport behavior sensitive to small variations. As material enters a constriction, confinement modifies velocity, pressure, diffusion, and passage time; repeated measurements can therefore reveal distributions, correlations, and outliers that statistical models quantify. In statistics, data from these systems support estimation of variability, comparison of experimental conditions, and evaluation of whether observed differences reflect meaningful effects or measurement noise. This analysis connects microscale physical behavior with reproducible conclusions in laboratory and engineering studies.

Micron Scale Constrictions - Related Videos

Research

JoVE Journal - Engineering
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Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System

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Cited by 6 •

2015

Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.

Research

JoVE Journal - Neuroscience
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Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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Cited by 20 •

2013

In this article we describe the full experimental procedure to reconstruct, with high resolution, the fine brain anatomy of fluorescently labeled mouse brains. The described protocol includes sample preparation and clearing, specimen mounting for imaging, data post-processing and multi-scale visualization.

Research

JoVE Journal - Bioengineering

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

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Cited by 3 •

2021

This work presents a bottom-up approach to the engineering of local magnetic forces for control of neuronal organization. Neuron-like cells loaded with magnetic nanoparticles (MNPs) are plated atop and controlled by a micro-patterned platform with perpendicular magnetization. Also described are magnetic characterization, MNP cellular uptake, cell viability, and statistical analysis.

Transverse Aortic Constriction in Mice

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Cited by 263 •

2010

Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model to study mechanisms underlying cardiac hypertrophy and heart failure development. Here, we describe procedures to constrict the aorta to create a reproducible degree of cardiac hypertrophy in mice.

Minimally Invasive Transverse Aortic Constriction in Mice

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Cited by 42 •

2017

Minimally invasive transverse aortic constriction (MTAC) conserves the essentials of regular transverse aortic constriction (TAC) while eliminating the use of a ventilator with tracheal intubation. It proves to be a highly desirable method for high-throughput studies on left ventricular overload, particularly in translational studies.

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