Dergi

/

/

Time-lapse Imaging of Mitosis After siRNA Transfection

JoVE Journal
Biyoloji

This content is Free Access.

JoVE Journal Biyoloji

Time-lapse Imaging of Mitosis After siRNA Transfection

Time-lapse Imaging of Mitosis After siRNA Transfection

16,712 Views

•

08:21 min

•

June 06, 2010

DOI:

DOI:

•

08:21 min

June 06, 2010

•

3 Views

Douglas R. Mackay¹, Katharine S. Ullman¹, Christopher K. Rodesch²

¹Department of Oncological Sciences, Huntsman Cancer Institute,Utah Üniversitesi, ²Fluorescence Microscopy Core Facility,Utah Üniversitesi

Özet

Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.

Explore More Videos

Time-lapse Imaging Mitosis SiRNA Transfection Cellular Organization Chromosome Dynamics Fluorescence Microscopy GFP-tagged Proteins RNAi-based Depletion Mitotic Regulatory Protein Automated Fluorescence Microscope Image Sequences Still-image Montages Movies Quantification Of Mitotic Stages MCherry-tagged Histone H2B Time-lapse Experiment Design

Read Article