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Dissection and Staining of Drosophila Larval Ovaries
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07:40 min
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May 13, 2011
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How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development. This video demonstrates how to dissect, stain and mount female gonads from late third instar (LL3) Drosophila larvae.
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DissectionStainingDrosophila Larval OvariesStem CellsDevelopmentEmbriyogenezMaintenanceRepairÇevreFruit FlyDrosophila MelanogasterGerm Line Stem Cells (GSCs)Somatic Support CellsNicheGenetic ManipulationInteractionsMechanismsDifferentiationSomatic NichesCellular ComponentsTerminal FilamentCap CellsLarval InstarPrimordial Germ Cells (PGCs)ProliferationFunctional UnitEggs
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Maimon, I., Gilboa, L. Dissection and Staining of Drosophila Larval Ovaries. J. Vis. Exp. (51), e2537, doi:10.3791/2537 (2011).
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