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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

16,837 Views

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09:40 min

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February 13, 2012

DOI:

10.3791/3442-v

DOI:
10.3791/3442-v

•

09:40 min
February 13, 2012

•

10 Views
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1Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

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We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.

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RNAi ScreeningC. ElegansPostembryonic PhenotypesModel SystemGenesGene PathwaysFunctional CharacterizationProtocolRNAi ScreenPhenotype AssayFluorescent ProductTransgeneGenomic RNAi LibraryCDNA InsertsRNAi-sensitive BackgroundHatched Animals

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Cite this Article

Beifuss, K. K., Gumienny, T. L. RNAi Screening to Identify Postembryonic Phenotypes in C. elegans. J. Vis. Exp. (60), e3442, doi:10.3791/3442 (2012).

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