High-resolution <em>In Vivo</em> Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Julie Winterburn; Jens C. Pruessner; Chavez Sofia; Mark M. Schira; Nancy J. Lobaugh; Aristotle N. Voineskos; M. Mallar Chakravarty

doi:10.3791/51861

Yüksek çözünürlük İn Vivo 3T Manyetik Rezonans Görüntüleme kullanma İnsan hipokampal ala...

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Neuroscience

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JoVE Journal Neuroscience

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Yüksek çözünürlük İn Vivo 3T Manyetik Rezonans Görüntüleme kullanma İnsan hipokampal alanlar için Manuel Segmentasyon Protokolü

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11:03 min

November 10, 2015

DOI: 10.3791/51861-v

Julie Winterburn^1,2, Jens C. Pruessner³, Chavez Sofia^4,5, Mark M. Schira^6,7, Nancy J. Lobaugh^4,8, Aristotle N. Voineskos^5,9, M. Mallar Chakravarty^1,2

¹Institute of Biomaterials and Biomedical Engineering,University of Toronto, ²Computational Brain Anatomy Laboratory, Douglas Institute,McGill University, ³McGill Centre for Studies in Aging,McGill University, ⁴MRI Unit, Research Imaging Centre, Campbell Family Mental Health Research Institute,Centre for Addiction and Mental Health, ⁵Department of Psychiatry,University of Toronto, ⁶School of Psychology,University of Wollongong, ⁷Neuroscience Research Australia, ⁸Department of Medicine,University of Toronto, ⁹Kimel Family Translational Imaging Genetics Research Laboratory, Research Imaging Centre, Campbell Family Mental Health Research Institute,Centre for Addiction and Mental Health

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Overview

This manuscript presents a protocol for segmenting the human hippocampus and its subfields using MRI. It focuses on providing an anatomically accurate method tailored for high-resolution images.

Key Study Components

Area of Science

Neuroimaging
Hippocampal anatomy
Magnetic resonance imaging

Background

The hippocampus is crucial for memory and spatial navigation.
Understanding its subfields can provide insights into neurological conditions.
High-resolution imaging is essential for accurate segmentation.
This study aims to enhance neuroimaging techniques for better analysis.

Purpose of Study

To develop a reliable method for segmenting hippocampal subfields.
To facilitate research on structural changes in healthy and diseased states.
To provide definitions for subfields along the entire hippocampal length.

Methods Used

Utilization of a three T MRI scanner for high-resolution imaging.
Segmentation of the hippocampus into five subfields: CA1, CA2/CA3, CA4/dentate gyrus, strata radiatum/lacunosum/moleculare, and subiculum.
Software interface for segmentation analysis.
Command-line instructions for initiating the segmentation process.

Main Results

The protocol allows for accurate segmentation of hippocampal subfields.
It provides a framework for analyzing structural changes in various conditions.
Results can enhance understanding of hippocampal function and pathology.
High-resolution images improve the reliability of the findings.

Conclusions

The developed protocol is a significant advancement in neuroimaging techniques.
It can aid in research related to memory disorders and other neurological conditions.
Future studies can build upon this method for further insights into hippocampal function.

Frequently Asked Questions

What is the significance of hippocampal subfields?

Hippocampal subfields play a critical role in memory and spatial navigation, and their study can reveal insights into neurological disorders.

How does this protocol improve neuroimaging?

This protocol provides a reliable and anatomically accurate method for segmenting the hippocampus, enhancing the quality of neuroimaging analysis.

What imaging technique is used in this study?

The study utilizes high-resolution magnetic resonance imaging (MRI) on a three T scanner.

Can this method be applied to other brain regions?

While this protocol focuses on the hippocampus, similar techniques could potentially be adapted for other brain regions.

What are the main advantages of this segmentation method?

The main advantages include anatomical accuracy, high-resolution imaging, and comprehensive subfield definitions.

How can this research impact clinical practices?

By improving the understanding of hippocampal structure, this research can inform diagnosis and treatment strategies for memory-related disorders.

Bu makalenin amacı, MRI kullanarak hipokampus ve hipokampal alt alanları incelemektir. El yazması, hipokampusu ve beş hipokampal alt yapıyı segmentlere ayırmak için bir protokolü tanımlar: cornu ammonis (CA) 1, CA2/CA3, CA4/dentate gyrus, strata radiatum/lacunosum/moleculare ve subiculum.

Bu protokolün genel amacı, insan hipokampusunu manyetik rezonans görüntülerindeki alt alanlarla bölümlere ayırmak için güvenilir, anatomik olarak doğru bir yöntem sağlamaktır. Burada, hipokampal alt alanlar beş etikete ayrılır: CA bir, CA iki, ca üç, CA dört, dentat girus, S-R-S-L-S-M ve icm. Bu yöntem, hipokampal alt alan yapısının sağlıklı ve hastalık durumlarında nasıl değiştiği gibi nörogörüntüleme alanındaki temel soruların yanıtlanmasına yardımcı olabilir.

Bu tekniğin ana avantajı, üç T tarayıcıda toplanan yüksek çözünürlüklü görüntülere göre uyarlanması ve hipokampusun tüm ön arka uzunluğu için alt alan tanımlarını içermesidir. Aşağıdaki komut sahnesini burada terminale girerek başlayın. Yazılım arayüzünü T bir ağırlıklı görüntüde açmak için koronal görünümü büyütün ve hipokampusu yakınlaştırın, gezinme penceresinde segmentlemeyi seçin ve ardından XY yarıçapını seçin.

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