Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Lara Stevanato; Caroline Hicks; John D. Sinden

doi:10.3791/52410

Dergi

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Gelişim Biyolojisi

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Üç Boyutlu Kültürleri, mıkroRNA Analizi ve Putatif Hedef Genler üzerinde bir İnsan Sinir Kök Hücre Hattı Farklılaşma

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

JoVE Journal
Gelişim Biyolojisi

This content is Free Access.

JoVE Journal Gelişim Biyolojisi

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Üç Boyutlu Kültürleri, mıkroRNA Analizi ve Putatif Hedef Genler üzerinde bir İnsan Sinir Kök Hücre Hattı Farklılaşma

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9,902 Views

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10:48 min

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April 12, 2015

DOI:

10.3791/52410-v

DOI:

10.3791/52410-v

•

10:48 min

April 12, 2015

•

18 Views

Lara Stevanato¹, Caroline Hicks¹, John D. Sinden¹

¹ReNeuron

Özet

With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.