Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Sylvia T. Cheung; Soroush Shakibakho; Eva Y. So; Alice L-F Mui

doi:10.3791/52807

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Lusiferaz haberci geni ile RAW264.7 hücreleri transfekte

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Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Lusiferaz haberci geni ile RAW264.7 hücreleri transfekte

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20,520 Views

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12:58 min

•

June 18, 2015

DOI:

10.3791/52807-v

DOI:

10.3791/52807-v

•

12:58 min

June 18, 2015

•

18 Views

Sylvia T. Cheung^1,2,3, Soroush Shakibakho^1,2, Eva Y. So^1,2, Alice L-F Mui^1,2,3

¹Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, ²Cerrahi Bölümü,University of British Columbia, ³Biyokimya ve Moleküler Biyoloji Anabilim Dalı,University of British Columbia

Özet

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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Lusiferaz haberci geni ile RAW264.7 hücreleri transfekte

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