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Memeli Hücrelerinde CRISPR / Cas9 aracılı Gen Knockout'larının Seçime Bağlı ve Bağımsız Üretimi
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
JoVE Journal
Genetik
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JoVE Journal Genetik
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Memeli Hücrelerinde CRISPR / Cas9 aracılı Gen Knockout'larının Seçime Bağlı ve Bağımsız Üretimi

Please note that all translations are automatically generated. Click here for the English version.
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12,537 Views

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11:35 min

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June 16, 2017

DOI:

10.3791/55903-v

DOI:
10.3791/55903-v

•

11:35 min
June 16, 2017

•

12468 Views
, ,
1Cell Biology and Neuroscience,Kaliforniya Üniversitesi, Riverside

Özet

Automatically generated

Somatik hücre dizilerini genetik olarak manipüle etme becerisindeki son gelişmeler, temel ve uygulamalı araştırmalar için büyük potansiyele sahiptir. Burada, CRISPR / Cas9'un ürettiği nakavt üretimi ve seçilebilir işaretlerin kullanılıp kullanılmadığı memeli hücre hatlarında tarama için iki yaklaşım sunuyoruz.

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Cite this Article

Sternburg, E. L., Dias, K. C., Karginov, F. V. Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. J. Vis. Exp. (124), e55903, doi:10.3791/55903 (2017).

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