Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Saikat Kumar B. Ghosh; Wayne B. Hunter; Alexis L. Park; Dawn E. Gundersen-Rindal

doi:10.3791/57390

Phloem ve Hemipteran böcekler bitki su besleme RNA müdahale ikna etmek için çift iplikçikli RNA O...

JoVE Journal
Environment

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Environment

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Phloem ve Hemipteran böcekler bitki su besleme RNA müdahale ikna etmek için çift iplikçikli RNA Oral teslim yöntemleri

Full Text

14,563 Views

10:14 min

May 4, 2018

DOI: 10.3791/57390-v

Saikat Kumar B. Ghosh¹, Wayne B. Hunter², Alexis L. Park¹, Dawn E. Gundersen-Rindal¹

¹Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service,United States Department of Agriculture, ²Horticultural Research Laboratory, Agricultural Research Service,United States Department of Agriculture

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects. The methods focus on delivering gene-specific dsRNAs to induce RNA interference in vivo and control insect pests.

Key Study Components

Area of Science

Plant biotechnology
Insect pest management
RNA interference

Background

RNA interference is a powerful tool for gene silencing.
Delivering biomolecules to insects presents significant challenges.
Hemipteran pests are major agricultural threats.
Novel delivery methods can enhance biopesticide efficacy.

Purpose of Study

To demonstrate a natural oral delivery method for dsRNA.
To elucidate the effects of RNAi-based molecular biopesticides.
To explore delivery methods applicable to various insect types.

Methods Used

Development of techniques for dsRNA delivery through plant vascular tissues.
Application of dsRNA to hemipteran pests.
Assessment of gene silencing effectiveness in vivo.
Evaluation of the method's applicability to other insect species.

Main Results

Successful delivery of dsRNA to target insect pests.
Induction of RNA interference observed in treated insects.
Potential for broader application to other sap-feeding and chewing insects.
Demonstrated effectiveness of RNAi-based biopesticides.

Conclusions

The oral delivery method shows promise for pest control.
RNAi can be effectively utilized in agricultural practices.
Further research is needed to optimize delivery techniques.

Frequently Asked Questions

What is RNA interference?

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, effectively silencing targeted genes.

How does the oral delivery method work?

The method involves delivering double-stranded RNA through plant vascular tissues, allowing it to reach sap-feeding insects effectively.

What types of insects can this method target?

The techniques demonstrated can target hemipteran pests, such as the brown marmorated stink bug and the Asian citrus psyllid, and may be applicable to other insect types.

What are the advantages of using RNAi-based biopesticides?

RNAi-based biopesticides can provide a targeted approach to pest control, reducing the impact on non-target organisms and the environment.

Is this method environmentally friendly?

Yes, RNAi-based methods can be more environmentally friendly compared to traditional chemical pesticides, as they specifically target pest genes.

What further research is needed?

Further research is needed to optimize the delivery techniques and assess the long-term effects of RNAi-based biopesticides in various agricultural settings.

Bu makalede damar doku bitkiler için RNA müdahale (RNAi) böcekler besleme phloem sap ile çift iplikçikli RNA (dsRNA) oral teslimat için geliştirilen roman tekniklerini gösterir.

Bu prosedürlerin genel amacı, doğal bir oral uygulama yöntemini göstermektir. Gene özgü dsRNA'lar için, in vivo ve böcek zararlılarında RNA girişimini indüklemek için. Bu yöntem, böceklerin içine biyomoleküllerin nasıl verileceği, RNAi bazlı Moleküler biyopestisitlerin etkisinin aydınlatılması ve çevredeki böceklerin kontrol edilmesi ile ilgili temel soruları yanıtlamaya yardımcı olabilir.

Bu tekniğin temel avantajı, nükleik asit aracılı gen susturma yoluyla bu dağıtımın, çiğneme böceklerinin yanı sıra bitki özsuyu beslemesine de uygulanabilmesidir. Buradaki yöntemler, çift sarmallı RNA'nın kahverengi zırhlı kokuşmuş böcek, alacalı böcek ve asya turunçgil psillidi gibi hemipteran zararlılara verildiğini gösterse de. Diğer böceklere de uygulanabilirler.

View the full transcript and gain access to thousands of scientific videos