Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Jaewook Kim; Myungji Kim; Dong Gyu Hwang; In Kyong Shim; Song Cheol Kim; Jinah Jang

doi:10.3791/60434

3D Hücre Yüklü Pankreas Dokusu Yapıları Baskı için Pankreas Doku Türetilmiş Ekstrasellüler Matrix...

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

3D Hücre Yüklü Pankreas Dokusu Yapıları Baskı için Pankreas Doku Türetilmiş Ekstrasellüler Matrix Bioink

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07:55 min

December 13, 2019

DOI: 10.3791/60434-v

Jaewook Kim*¹, Myungji Kim*², Dong Gyu Hwang², In Kyong Shim³, Song Cheol Kim^3,4, Jinah Jang^1,2,5

¹Department of Mechanical Engineering,Pohang University of Science and Technology, ²School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology, ³Asan Institute for Life Science,University of Ulsan College of Medicine and Asan Medical Center, ⁴Division of Hepato-Biliary and Pancreatic Surgery, Department of Surgery,University of Ulsan College of Medicine and Asan Medical Center, ⁵Department of Creative IT Engineering,Pohang University of Science and Technology

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Overview

This article discusses the use of decellularized extracellular matrix (dECM) bioink for creating 3D pancreatic tissue constructs. It highlights the protocols for decellularizing pancreatic tissue and the benefits of using pdECM bioink for islet encapsulation.

Key Study Components

Area of Science

Bioprinting
Tissue Engineering
Pancreatic Biology

Background

Decellularized extracellular matrix (dECM) mimics the natural tissue environment.
pdECM bioink preserves tissue-specific components and architecture.
Islet encapsulation is crucial for diabetes treatment.
3D constructs can enhance the interaction between islets and surrounding tissues.

Purpose of Study

To develop protocols for decellularizing pancreatic tissue.
To evaluate the properties of pancreatic tissue-derived dECM bioink.
To create transplantable pancreatic tissue constructs for type 1 diabetes treatment.

Methods Used

Decellularization of frozen porcine pancreas tissue.
Preparation of pdECM bioink for 3D bioprinting.
Assessment of bioink properties for cell viability and printability.
Fabrication of 3D pancreatic tissue constructs.

Main Results

pdECM bioink provides an optimal microenvironment for pancreatic islets.
Encapsulated islets show reduced cell death and improved functionality.
The technique allows for the preservation of tissue-specific composition.
Applications extend to diabetes treatment and pancreatic cancer models.

Conclusions

pdECM bioink is effective for creating transplantable pancreatic constructs.
This approach enhances the potential for diabetes therapies.
Further research could expand its use in other tissue engineering applications.

Frequently Asked Questions

What is dECM?

Decellularized extracellular matrix (dECM) is a biomaterial derived from tissues that retains the natural architecture and biochemical cues of the original tissue.

How is pdECM bioink prepared?

pdECM bioink is prepared by decellularizing pancreatic tissue and processing it to maintain its tissue-specific properties.

What are the benefits of using pdECM bioink?

pdECM bioink provides a supportive microenvironment for cell survival and function, enhancing the viability of encapsulated islets.

What applications does this research have?

This research can be applied to the development of transplantable pancreatic tissue constructs and in vitro models for diabetes and pancreatic cancer.

What is the significance of 3D bioprinting in this study?

3D bioprinting allows for the precise fabrication of tissue constructs that can mimic the natural architecture of pancreatic tissues.

Can this technique be used for other types of tissues?

Yes, the principles of using dECM bioink can potentially be adapted for other tissues beyond the pancreas.

Hücre dışı matriks (dECM) tasarlanmış bir yapıda hedef dokuların doğal işlevlerini yeniden özetlemek için uygun mikroçevresel ipuçları sağlayabilir. Bu makalede pankreas dokusunun desellülsizasyonu için protokoller açıklanmıştır, pankreas dokusu kaynaklı dECM bioink decm dECM değerlendirilmesi, ve 3D pankreas dokusu üretimi bir biyobaskı tekniği kullanılarak inşa eder.

pdECM bioink dokuya özgü bileşenleri ve mimarisi kullanarak pankreas adacıkları için yararlı bir mikroortam sağlayabilir ve hücre ölümlerini azaltmak ve yazdırılabilirliğini artırmak optimal nörolojik özelliklere sahiptir. Bu tekniğin ana avantajı dokuya özgü kompozisyon 3D pankreas doku yapıları ve kapsüllü adacıklar arasında yapıcı bir crosstalk teşvik pdECM bioink içinde korunabilir olmasıdır. pdECM bioink adacık kapsülleme gelişimi tip bir diyabet tedavisi için transplante edilebilir pankreas dokusu yapılması için uygulanabilir.

Bu bioink nakledilebilir yapılar yanı sıra diyabet, diyabete bağlı komplikasyonlar ve pankreas kanseri için in vitro doku modellerinin uygulanmasını genişletmek için kullanılabilir. Dondurulmuş domuz pankreas doku örneğinin hücreden arındırılması için, bir milimetre kalınlığında parçalar halinde dondurulmuş pankreas dilim ve 500 mililitrelik plastik konteyner içine dilimlenmiş doku 50 gram aktarmak için bir rende kullanın. Yaklaşık 12 saat boyunca dakikada 150 rotasyonlarda dijital orbital shaker üzerinde dört santigrat derece distile su 300 mililitre ile doku yıkayın.

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