Kolon Kanseri Kök Hücrelerini İncelemek İçin Üç Boyutlu Bir Küresel Model
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January 22, 2021
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Bu protokol, Caco2 kolon adenokarsinom hücrelerinden üç boyutlu küreseller üretmek ve yetiştirmek için yeni, sağlam ve tekrarlanabilir bir kültür sistemi sunar. Sonuçlar, kemoterapiye yanıt da dahil olmak üzere kanser kök hücre biyolojisini incelemek için bu yaklaşımın uygunluğu için ilk kavram kanıtını sağlar.
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Cite this Article
Giolito, M. V., Claret, L., Frau, C., Plateroti, M. A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells. J. Vis. Exp. (167), e61783, doi:10.3791/61783 (2021).
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