CRISPR/Cas9 Mutagenesis için Kum Sineği (Phlebotomus papatasi) Embriyo Mikroenjeksiyonu
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November 17, 2020
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Bu protokol, CRISPR/Cas9 hedefli mutajenizin kum sineklerindeki adımlarını detaylandırıyor: embriyo toplama, enjeksiyon, böcek yetiştirme ve tanımlamanın yanı sıra ilgi çekici mutasyonların seçimi.
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Cite this Article
Louradour, I., Ghosh, K., Inbar, E., Sacks, D. L., Aluvihare, C., Harrell II, R. A. Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis. J. Vis. Exp. (165), e61924, doi:10.3791/61924 (2020).
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