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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

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07:19 min

February 02, 2021

DOI:

07:19 min
February 02, 2021

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Özet

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Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

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