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De novo Aktif Olarak Çevrilmiş Açık Okuma Çerçevelerinin Ribozom Profil Oluşturma Verileri ile Tanımlanması
<em>De novo</em> Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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JoVE Journal Biyoloji
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Aktif Olarak Çevrilmiş Açık Okuma Çerçevelerinin Ribozom Profil Oluşturma Verileri ile Tanımlanması

Please note that all translations are automatically generated. Click here for the English version.
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3,548 Views

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08:23 min

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February 18, 2022

DOI:

10.3791/63366-v

DOI:
10.3791/63366-v

•

08:23 min
February 18, 2022

•

3489 Views
, , ,
1School of Basic Medical Sciences,Xi’an Jiaotong University Health Science Center2MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences,Tsinghua University3Joint Graduate Program of Peking-Tsinghua-National Institute of Biological Science

Özet

Automatically generated

Ribozomların çevrilmesi, kodon başına üç nükleotidin peptitlere şifresini çözer. Ribozom profillemesi ile yakalanan mRNA boyunca hareketleri, karakteristik üçlü periyodiklik sergileyen ayak izlerini üretir. Bu protokol, tüm transkriptom düzeyinde aktif olarak çevrilmiş açık okuma çerçevelerini tanımlamak için ribozom profil oluşturma verilerinden bu belirgin özelliği deşifre etmek için RiboCode'un nasıl kullanılacağını açıklar.

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Cite this Article

Zhu, Y., Li, F., Yang, X., Xiao, Z. De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data. J. Vis. Exp. (180), e63366, doi:10.3791/63366 (2022).

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