A Mouse Model of Ankle-Subtalar Complex Joint Instability

Shuo Wang; Peixin Liu; Chunzhuo Hua; Hongtao Zhang; Jia Yu

doi:10.3791/64481

Ayak Bileği Subtalar Kompleks Eklem İnstabilitesinin Bir Fare Modeli

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Biology

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JoVE Journal Biology

A Mouse Model of Ankle-Subtalar Complex Joint Instability

Ayak Bileği Subtalar Kompleks Eklem İnstabilitesinin Bir Fare Modeli

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09:14 min

October 28, 2022

DOI: 10.3791/64481-v

Shuo Wang*^1,2, Peixin Liu*^1,2, Chunzhuo Hua^1,2, Hongtao Zhang^1,2, Jia Yu^1,2

¹Department of Orthopaedics,The First Affiliated Hospital of Soochow University, ²Orthopaedic Institute,Soochow University

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Overview

The study focuses on the ankle-subtalar complex joint (ASCJ), essential for balance control during daily activities. It presents a mouse model of ligament transection-induced instability in the ASCJ, providing insights for clinical foot disease research.

Key Study Components

Research Area

Orthopedic research
Biomechanics
Animal models for foot diseases

Background

The ASCJ's role in foot stability and injury response.
Importance of accurate animal models for clinical relevance.
Understanding sports-related joint instability.

Methods Used

Mouse model of ligament transection
Balance and gait training assessments
Histological examination of joint architecture

Main Results

Successful establishment of a clinically relevant ASCJ instability model.
Assessment of gait performance and balance post-surgery.
Histological verification of joint structure post-dissection.

Conclusions

The model enhances understanding of foot joint instability.
It provides a platform for studying diagnosis and treatment strategies for foot diseases.

Frequently Asked Questions

What is the ASCJ and why is it important?

The ankle-subtalar complex joint (ASCJ) is critical for maintaining balance and stability during movement.

How does ligament transection affect the ASCJ?

Ligament transection leads to instability in the ASCJ, affecting gait and balance.

What methods were used to assess the animals in this study?

The study employed balance beam tests and gait training to evaluate animal performance.

Why is a mouse model used for studying foot diseases?

Mice provide a controlled environment suitable for replicating human foot conditions and studying pathophysiological mechanisms.

What are the implications of this research?

This research aims to inform clinical approaches to diagnosing and treating foot diseases stemming from joint instability.

How does this model contribute to future research?

It allows for further investigation into mechanistic aspects of ankle instability and potential therapeutic interventions.

What histological assessments were performed in the study?

Histological examinations were conducted to observe structural changes in the ASCJ post-surgery, verifying joint integrity.

Ayak bileği-subtalar kompleks eklem (ASCJ) ayağın çekirdeğidir ve günlük aktivitelerde denge kontrolünde önemli bir rol oynar. Spor yaralanmaları genellikle bu eklemde dengesizliğe yol açar. Burada, ASCJ'nin bağ transeksiyonuna bağlı instabilitesinin bir fare modelini açıklıyoruz.

ASCJ yetersizlik fare modelinin başarılı bir şekilde kurulması, basit bir ayak bileği yetersizliği hayvan modelini genişletir ve klinik ayak hastalıklarının incelenmesi için yeni kavramlar sağlar. Ayak hastalıklarının teşhisi ve tedavisi hakkında daha fazla araştırma yapmak için kullanılabilecek gerçek klinik durumla daha uyumlu bir hayvan modeli oluşturduk. Deneyden bir hafta önce, fareleri denge kirişi ve yürüyüş eğitimine tabi tutun, bir denge kirişinin veya U şeklindeki borunun bir ucundan diğerine durmadan gidin.

Farenin sağ arka bacaklarının ayak bileği eklemindeki kılları uyuşturduktan ve çıkardıktan sonra, açıkta kalan cildi bir iyot çubuğu ile dezenfekte edin. Steril bir cerrahi ped kullanarak fareyi mikrocerrahi hayvan ameliyathanesine aktarın. Transvers servikal ve anterior talofibular ligament grupları için, sağ ayak bileği ekleminin üzerindeki deride bir neşter ile yedi milimetre eğik aşağı doğru uzunlamasına bir kesi yapın.

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