Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Olivia Graham; Jeimmy Rodriguez; Lillian van Biljon; Bisiayo Fashemi; Emily Graham; Katherine Fuh; Dineo Khabele; Mary Mullen

doi:10.3791/64878

Yüksek Dereceli Seröz Yumurtalık Kanseri Hasta Kaynaklı Organoidlerin Üretimi ve Kültürlenmesi

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Yüksek Dereceli Seröz Yumurtalık Kanseri Hasta Kaynaklı Organoidlerin Üretimi ve Kültürlenmesi

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07:19 min

January 6, 2023

DOI: 10.3791/64878-v

Olivia Graham*¹, Jeimmy Rodriguez*¹, Lillian van Biljon¹, Bisiayo Fashemi¹, Emily Graham¹, Katherine Fuh^1,2, Dineo Khabele¹, Mary Mullen¹

¹Washington University in St. Louis, ²University of California San Francisco

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Overview

Patient-derived organoids (PDO) are three-dimensional cultures that accurately mimic the tumor environment in vitro, particularly in high-grade serous ovarian cancer. These models are essential for studying novel biomarkers and therapeutics.

Key Study Components

Area of Science

Neuroscience
Oncology
Cell Biology

Background

PDOs represent a reliable model for studying ovarian cancer.
They recapitulate clonal heterogeneity and the tumor microenvironment.
PDOs overcome limitations of traditional cancer cell lines and patient-derived xenografts.
These organoids offer long-term expansion and mimic tumor physiology.

Purpose of Study

To study novel therapeutics in ovarian cancer.
To identify predictive biomarkers.
To understand complex biological processes in cancer.

Methods Used

Harvesting organoids from tumor samples.
Mincing tissue to create a homogenous mixture.
Using a dissociation tube for processing.
In vitro culture techniques for organoid maintenance.

Main Results

PDOs effectively mimic the tumor environment.
They allow for the study of therapeutic responses.
PDOs maintain genetic and functional characteristics of parent tumors.
They provide insights into tumor biology and treatment efficacy.

Conclusions

PDOs are ideal for studying ovarian cancer therapeutics.
They represent a significant advancement over traditional models.
These models can lead to better understanding and treatment of ovarian cancer.

Frequently Asked Questions

What are patient-derived organoids?

Patient-derived organoids are 3D cultures that mimic the tumor environment, allowing for the study of cancer biology and therapeutics.

How are organoids harvested?

Organoids are harvested by mincing tumor samples and processing them into a homogenous mixture for culture.

What advantages do PDOs have over traditional cancer models?

PDOs recapitulate tumor heterogeneity and microenvironment, providing a more accurate model for research.

Can PDOs be used for long-term studies?

Yes, PDOs offer long-term expansion and storage capabilities for ongoing research.

What types of cancer can PDOs be used to study?

PDOs can be used to study various cancers, including ovarian cancer, by mimicking the tumor physiology.

Are PDOs effective for testing new therapies?

Yes, PDOs are effective for testing novel therapeutics and understanding their impact on tumor biology.

Hasta kaynaklı organoidler (PDO), tümör ortamını in vitro olarak taklit edebilen üç boyutlu (3D) bir kültürdür. Yüksek dereceli seröz over kanserinde, PDO'lar yeni biyobelirteçleri ve terapötikleri incelemek için bir model oluşturur.

Hasta sürücü organoid modelleri, genetik ve fonksiyonel olarak ana tümörlerini temsil eder ve bu nedenle yumurtalık kanserinde yeni terapötikleri ve karmaşık biyolojik süreçleri incelemek için güvenilir modellerdir. Klonal heterojenliği, tümör mikroçevresini ve in vitro hücreden hücreye etkileşimleri özetleyerek, bu modeller kanser hücre hatlarının ve hasta kaynaklı ksenogreftlerin sınırlamalarının üstesinden gelir. Hasta kaynaklı organoidler uzun süreli genişleme ve depolama yetenekleri sunar ve tümör fizyolojisini taklit eder.

Sonuç olarak, yeni terapötiklerin ve öngörücü biyobelirteçlerin incelenmesi için ideal modellerdir. Tümör örneklerini 10 santimetrelik bir doku kültürü kabına yerleştirerek ve tek kullanımlık bir neşter kullanarak homojen bir karışım oluşturmak için dokuyu kıyarak organoidlerin toplanmasına başlayın. Homojen doku karışımını forseps kullanarak bir ayrışma tüpüne yerleştirin.

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