Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

1.6K görüntüleme

2 kez atıf

05:59 dk

3 Mayıs 2024

10.3791/66784-v

3 Mayıs 2024

1.6K görüntüleme

The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.

Daha fazla video keşfet

Nuclei Isolation

Chapters in this video

0:00

Introduction

2:05

Pulverization and Homogenization of Frozen Human Intermuscular Adipose Tissue Samples for Nuclei Isolation

3:41

Single‐Cell Nuclei Isolation from Homogenized Human Intermuscular Adipose Tissue Samples for RNA Sequencing

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