Name: İnsan Renal Arteriyel Dallarının Çok Düzeyli Mikrodiseksiyonu ve Fonksiyonel-Yapısal Profillemesi (Video) | JoVE Journal
Uploaded: 2025-09-05T13:00:00.000+00:00
Duration: 6 min 51 s
Description: Keşfedin, insan renal arteriyel dallarının mikrodiseksiyonu ve tel miyografi ile fonksiyonel değerlendirmesini. Bu yöntem, vasküler disfonksiyon mekanizmalarını anlamaya ve hedefe yönelik tedaviler geliştirmeye yardımcı olur.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Molema, G., Aird, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+heterogeneity+in+the+kidney%5BTitle%5D)+AND+%22Semin+Nephrol%22%5BJournal%5D)">Vascular heterogeneity in the kidney.</a> Semin Nephrol. 32 (2), 145-155 (2012).</li>
<li>Jentzer, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Contemporary+management+of+severe+acute+kidney+injury+and+refractory+cardiorenal+syndrome%3A+Jacc+council+perspectives%5BTitle%5D)+AND+%22J+Am+Coll+Cardiol%22%5BJournal%5D)">Contemporary management of severe acute kidney injury and refractory cardiorenal syndrome: Jacc council perspectives.</a> J Am Coll Cardiol. 76 (9), 1084-1101 (2020).</li>
<li>Kanji, H. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Difference+between+preoperative+and+cardiopulmonary+bypass+mean+arterial+pressure+is+independently+associated+with+early+cardiac+surgeryassociated+acute+kidney+injury%5BTitle%5D)+AND+%22J+Cardiothorac+Surg%22%5BJournal%5D)">Difference between preoperative and cardiopulmonary bypass mean arterial pressure is independently associated with early cardiac surgeryassociated acute kidney injury.</a> J Cardiothorac Surg. 5 (1), 71(2010).</li>
<li>Kwiatkowska, E., Kwiatkowski, S., Dziedziejko, V., Tomasiewicz, I., Domanski, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+microcirculation+injury+as+the+main+cause+of+ischemic+acute+kidney+injury+development%5BTitle%5D)+AND+%22Biology+%28Basel%29%22%5BJournal%5D)">Renal microcirculation injury as the main cause of ischemic acute kidney injury development.</a> Biology (Basel). 12 (2), 327(2023).</li>
<li>Ruilope, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hypertension+in+2010%3ABlood+pressure+and+the+kidney%5BTitle%5D)+AND+%22Nat+Rev+Nephrol%22%5BJournal%5D)">Hypertension in 2010:Blood pressure and the kidney.</a> Nat Rev Nephrol. 7 (2), 73-74 (2011).</li>
<li>Barger, A. C., Herd, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+renal+circulation%5BTitle%5D)+AND+%22N+Engl+J+Med%22%5BJournal%5D)">The renal circulation.</a> N Engl J Med. 284 (9), 482-490 (1971).</li>
<li>Beloncle, F., Piquilloud, L., Asfar, P. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aBeloncle%2C+F+%22Critical+care+nephrology%22">Critical care nephrology</a>. , third edition, Elsevier. Philadelphia. (2019).</li>
<li>MompeóCorredera, B., HernándezMorera, P., CastañoGonzález, I., QuintanaMontesdeoca, M. D. P., MederosReal, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Regions+of+the+human+renal+artery%3AHistomorphometric+analysis%5BTitle%5D)+AND+%22Anat+Cell+Biol%22%5BJournal%5D)">Regions of the human renal artery:Histomorphometric analysis.</a> Anat Cell Biol. 55 (3), 330-340 (2022).</li>
<li>Evans, R. G., Smith, D. W., Lee, C. J., Ngo, J. P., Gardiner, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((What+makes+the+kidney+susceptible+to+hypoxia%5BTitle%5D)+AND+%22Anat+Rec+%28Hoboken%29%22%5BJournal%5D)">What makes the kidney susceptible to hypoxia.</a> Anat Rec (Hoboken). 303 (10), 2544-2552 (2020).</li>
<li>Hwa, J. J., Ghibaudi, L., Williams, P., Chatterjee, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+acetylcholinedependent+relaxation+in+large+and+small+arteries+of+rat+mesenteric+vascular+bed%5BTitle%5D)+AND+%22Am+J+Physiol%22%5BJournal%5D)">Comparison of acetylcholinedependent relaxation in large and small arteries of rat mesenteric vascular bed.</a> Am J Physiol. 266 (3 Pt 2), H952-H958 (1994).</li>
<li>Buus, N. H., Van Bavel, E., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differences+in+sensitivity+of+rat+mesenteric+small+arteries+to+agonists+when+studied+as+ring+preparations+or+as+cannulated+preparations%5BTitle%5D)+AND+%22Br+J+Pharmacol%22%5BJournal%5D)">Differences in sensitivity of rat mesenteric small arteries to agonists when studied as ring preparations or as cannulated preparations.</a> Br J Pharmacol. 112 (2), 579-587 (1994).</li>
<li>Barone, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+arterial+and+venous+doppler+in+cardiorenal+syndrome%3A+Pathophysiological+and+clinical+insights%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">Renal arterial and venous doppler in cardiorenal syndrome: Pathophysiological and clinical insights.</a> Biomedicines. 12 (6), 1166(2024).</li>
<li>Cuttone, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exploring+the+utility+of+renal+resistive+index+in+critical+care%3A+Insights+into+ARDS+and+cardiac+failure%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">Exploring the utility of renal resistive index in critical care: Insights into ARDS and cardiac failure.</a> Biomedicines. 13 (2), 519(2025).</li>
<li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Clinical+practice+report+of+contrastenhanced+ultrasound+in+renal+artery+disease%5BTitle%5D)+AND+%22J+Ultrasound+Med%22%5BJournal%5D)">Clinical practice report of contrastenhanced ultrasound in renal artery disease.</a> J Ultrasound Med. 43 (1), 117-125 (2023).</li>
<li>Andersen, F. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Continuous+assessment+of+tissue+perfusion+using+quantitative+indocyanine+green+fluorescence+imaging+during+controlled+hypo+and+reperfusion%5BTitle%5D)+AND+%22Surg+Endosc%22%5BJournal%5D)">Continuous assessment of tissue perfusion using quantitative indocyanine green fluorescence imaging during controlled hypo and reperfusion.</a> Surg Endosc. 39 (10), 6512-6521 (2025).</li>
<li>Schubert, R., Gaynullina, D., Shvetsova, A., Tarasova, O. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myography+of+isolated+blood+vessels%3AConsiderations+for+experimental+design+and+combination+with+supplementary+techniques%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Myography of isolated blood vessels:Considerations for experimental design and combination with supplementary techniques.</a> Front Physiol. 14 (8), 1176748(2023).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mir1865p+carried+by+M2+macrophagederived+exosomes+downregulates+TRPP2+expression+in+airway+smooth+muscle+to+alleviate+asthma+progression%5BTitle%5D)+AND+%22Int+Immunopharmacol%22%5BJournal%5D)">Mir1865p carried by M2 macrophagederived exosomes downregulates TRPP2 expression in airway smooth muscle to alleviate asthma progression.</a> Int Immunopharmacol. 148 (1), 114107(2025).</li>
<li>Ikeda, M., Nakada, A., Abukawa, H., Yamazaki, T., Maruyama, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vibegron+inhibits+enhanced+spontaneous+contractions+induced+by+anoxia%2Freoxygenation+in+isolated+whole+bladder+from+rats%5BTitle%5D)+AND+%22Eur+J+Pharmacol%22%5BJournal%5D)">Vibegron inhibits enhanced spontaneous contractions induced by anoxia/reoxygenation in isolated whole bladder from rats.</a> Eur J Pharmacol. 926 (1), 175017(2022).</li>
<li>Wenceslau, C. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Guidelines+for+the+measurement+of+vascular+function+and+structure+in+isolated+arteries+and+veins%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Guidelines for the measurement of vascular function and structure in isolated arteries and veins.</a> Am J Physiol Heart Circ Physiol. 321 (1), H77-H111 (2021).</li>
<li>Bayliss, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((On+the+local+reactions+of+the+arterial+wall+to+changes+of+internal+pressure%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">On the local reactions of the arterial wall to changes of internal pressure.</a> J Physiol. 28 (3), 220-231 (1902).</li>
<li>Darmon, M., Schnell, D., Schneider, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Monitoring+of+renal+perfusion%5BTitle%5D)+AND+%22Intensive+Care+Med%22%5BJournal%5D)">Monitoring of renal perfusion.</a> Intensive Care Med. 48 (10), 1505-1507 (2022).</li>
<li>Offenhäuser, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((EphA2+regulates+vascular+permeability+and+prostate+cancer+metastasis+via+modulation+of+cell+junction+protein+phosphorylation%5BTitle%5D)+AND+%22Oncogene%22%5BJournal%5D)">EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylation.</a> Oncogene. 44 (4), 208-227 (2025).</li>
<li>Vitverova, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Soluble+endoglin+and+hypercholesterolemia+aggravate+endothelial+and+vessel+wall+dysfunction+in+mouse+aorta%5BTitle%5D)+AND+%22Atherosclerosis%22%5BJournal%5D)">Soluble endoglin and hypercholesterolemia aggravate endothelial and vessel wall dysfunction in mouse aorta.</a> Atherosclerosis. 271, 15-25 (2018).</li>
<li>Maathuis, M. H. J., De Groot, M., Ploeg, R. J., Leuvenink, H. G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deterioration+of+endothelial+and+smooth+muscle+cell+function+in+DCD+kidneys+after+static+cold+storage+in+IGL1+or+UW%5BTitle%5D)+AND+%22J+Surg+Res%22%5BJournal%5D)">Deterioration of endothelial and smooth muscle cell function in DCD kidneys after static cold storage in IGL1 or UW.</a> J Surg Res. 152 (2), 231-237 (2009).</li>
<li>Ingemansson, R., Budrikis, A., Bolys, R., Sjoberg, T., Steen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+temperature+in+longterm+preservation+of+vascular+endothelial+and+smooth+muscle+function%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Effect of temperature in longterm preservation of vascular endothelial and smooth muscle function.</a> Ann Thorac Surg. 61 (5), 1413-1417 (1996).</li>
<li>McIntyre, C. A., Williams, B. C., Lindsay, R. M., McKnight, J. A., Hadoke, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preservation+of+vascular+function+in+rat+mesenteric+resistance+arteries+following+cold+storage%2C+studied+by+small+vessel+myography%5BTitle%5D)+AND+%22Br+J+Pharmacol%22%5BJournal%5D)">Preservation of vascular function in rat mesenteric resistance arteries following cold storage, studied by small vessel myography.</a> Br J Pharmacol. 123 (8), 1555-1560 (1998).</li>
<li>Ingemansson, R., Sjoberg, T., Massa, G., Steen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Longterm+preservation+of+vascular+endothelium+and+smooth+muscle%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Longterm preservation of vascular endothelium and smooth muscle.</a> Ann Thorac Surg. 59 (5), 1177-1181 (1995).</li>
<li>Zheng, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HMGB2+promotes+smooth+muscle+cell+proliferation+through+PPAR%CE%B3%2FPGC1%CE%B1+pathwaymediated+glucose+changes+in+aortic+dissection%5BTitle%5D)+AND+%22Atherosclerosis%22%5BJournal%5D)">HMGB2 promotes smooth muscle cell proliferation through PPARγ/PGC1α pathwaymediated glucose changes in aortic dissection.</a> Atherosclerosis. 399, 119044(2024).</li>
<li>Zhang, B., Zhang, Y., Deng, F., Fang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ligustrazine+prevents+basilar+artery+remodeling+in+twokidneytwoclip+renovascular+hypertension+rats+via+suppressing+PI3K%2FAKT+signaling%5BTitle%5D)+AND+%22Microvasc+Res%22%5BJournal%5D)">Ligustrazine prevents basilar artery remodeling in twokidneytwoclip renovascular hypertension rats via suppressing PI3K/AKT signaling.</a> Microvasc Res. 128, 103938(2020).</li>
<li>Somiari, S. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+the+quality+of+RNA+isolated+from+human+breast+tissue+after+ambient+room+temperature+exposure%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure.</a> PLoS One. 17 (1), e0262654(2022).</li>
<li>Srinivasan, M., Sedmak, D., Jewell, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+fixatives+and+tissue+processing+on+the+content+and+integrity+of+nucleic+acids%5BTitle%5D)+AND+%22Am+J+Pathol%22%5BJournal%5D)">Effect of fixatives and tissue processing on the content and integrity of nucleic acids.</a> Am J Pathol. 161 (6), 1961-1971 (2002).</li>
<li>Potente, M., Makinen, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+heterogeneity+and+specialization+in+development+and+disease%5BTitle%5D)+AND+%22Nat+Rev+Mol+Cell+Biol%22%5BJournal%5D)">Vascular heterogeneity and specialization in development and disease.</a> Nat Rev Mol Cell Biol. 18 (8), 477-494 (2017).</li>
<li>Lake, B. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+atlas+of+healthy+and+injured+cell+states+and+niches+in+the+human+kidney%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">An atlas of healthy and injured cell states and niches in the human kidney.</a> Nature. 619 (7970), 585-594 (2023).</li>
<li>Greenbaum, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+spatially+resolved+timeline+of+the+human+maternal-fetal+interface%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">A spatially resolved timeline of the human maternal-fetal interface.</a> Nature. 619 (7970), 595-605 (2023).</li>
<li>The Tabula Sapiens Consortium. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Tabula+Sapiens%3A+A+multipleorgan%2C+singlecell+transcriptomic+atlas+of+humans%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">The Tabula Sapiens: A multipleorgan, singlecell transcriptomic atlas of humans.</a> Science. 376 (6594), eabl4896(2022).</li>
<li>Barnett, S. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+organotypic+atlas+of+human+vascular+cells%5BTitle%5D)+AND+%22Nat+Med%22%5BJournal%5D)">An organotypic atlas of human vascular cells.</a> Nat Med. 30 (12), 3468-3481 (2024).</li>
<li>Shen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeting+cytokinelike+protein+FAM3D+lowers+blood+pressure+in+hypertension%5BTitle%5D)+AND+%22Cell+Rep+Med%22%5BJournal%5D)">Targeting cytokinelike protein FAM3D lowers blood pressure in hypertension.</a> Cell Rep Med. 4 (6), 101072(2023).</li>
<li>Zhao, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Restoration+of+autophagic+flux+improves+endothelial+function+in+diabetes+through+lowering+mitochondrial+ROSmediated+eNOS+monomerization%5BTitle%5D)+AND+%22Diabetes%22%5BJournal%5D)">Restoration of autophagic flux improves endothelial function in diabetes through lowering mitochondrial ROSmediated eNOS monomerization.</a> Diabetes. 71 (5), 1099-1114 (2022).</li>
<li>Ergin, B., Akin, S., Ince, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Kidney+microcirculation+as+a+target+for+innovative+therapies+in+AKI%5BTitle%5D)+AND+%22J+Clin+Med%22%5BJournal%5D)">Kidney microcirculation as a target for innovative therapies in AKI.</a> J Clin Med. 10 (18), (2021).</li>
<li>Deferrari, G., Cipriani, A., La Porta, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Renal+dysfunction+in+cardiovascular+diseases+and+its+consequences%5BTitle%5D)+AND+%22J+Nephrol%22%5BJournal%5D)">Renal dysfunction in cardiovascular diseases and its consequences.</a> J Nephrol. 34 (1), 137-153 (2021).</li>
<li>Owen, M. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue+potentiates+contraction+of+coronary+vascular+smooth+muscle%3A+Influence+of+obesity%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Perivascular adipose tissue potentiates contraction of coronary vascular smooth muscle: Influence of obesity.</a> Circulation. 128 (1), 9-18 (2013).</li>
<li>AyalaLopez, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue+contains+functional+catecholamines%5BTitle%5D)+AND+%22Pharmacol+Res+Perspect%22%5BJournal%5D)">Perivascular adipose tissue contains functional catecholamines.</a> Pharmacol Res Perspect. 2 (3), e00041(2014).</li>
<li>AyalaLopez, N., Thompson, J. M., Watts, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipose+tissue%27s+impact+on+norepinephrineinduced+contraction+of+mesenteric+resistance+arteries%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Perivascular adipose tissue's impact on norepinephrineinduced contraction of mesenteric resistance arteries.</a> Front Physiol. 8, 37(2017).</li>
<li>Ahmad, M. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perivascular+adipocytes+store+norepinephrine+by+vesicular+transport%5BTitle%5D)+AND+%22Arterioscler+Thromb+Vasc+Biol%22%5BJournal%5D)">Perivascular adipocytes store norepinephrine by vesicular transport.</a> Arterioscler Thromb Vasc Biol. 39 (2), 188-199 (2019).</li>
<li>Ya-Jing, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adipose+tissue-derived+cytokines%2C+CTRPs+as+biomarkers+and+therapeutic+targets+in+metabolism+and+the+cardiovascular+system%5BTitle%5D)+AND+%22Vessel+Plus%22%5BJournal%5D)">Adipose tissue-derived cytokines, CTRPs as biomarkers and therapeutic targets in metabolism and the cardiovascular system.</a> Vessel Plus. (1), 202-212 (2017).</li>
<li>Osol, G., Brekke, J. F., McElroyYaggy, K., Gokina, N. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myogenic+tone%2C+reactivity%2C+and+forced+dilatation%3A+A+threephase+model+of+in+vitro+arterial+myogenic+behavior%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Myogenic tone, reactivity, and forced dilatation: A threephase model of in vitro arterial myogenic behavior.</a> Am J Physiol Heart Circ Physiol. 283 (6), H2260-H2267 (2002).</li>
</ol>

<table><tbody><tr><td>Asetilkolin klorür</td><td>Sigma-Aldrich, Merck, Darmstadt, Almanya</td><td>A6625 Serisi</td><td></td></tr><tr><td>Siyah tabanlı kültür tabağı</td><td>Danimarka Myo Technology, Aarhus, Danimarka</td><td>300412</td><td></td></tr><tr><td>CaCl2</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A501330</td><td></td></tr><tr><td>D-glikoz</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A610219</td><td></td></tr><tr><td>Dumont forseps</td><td>Danimarka Myo Technology, Aarhus, Danimarka</td><td>300413</td><td></td></tr><tr><td>Geuder diseksiyon makası</td><td>Danimarka Myo Technology, Aarhus, Danimarka</td><td>400431</td><td></td></tr><tr><td>Kılavuz tel</td><td>Danimarka Myo Technology, Aarhus, Danimarka</td><td>400447</td><td>Çap 40 &mikro; m</td></tr><tr><td>Kartal</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A100395</td><td></td></tr><tr><td>KH2PO4</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A100781</td><td></td></tr><tr><td>LabChart Professional sürüm 8.3&nbsp;</td><td>ADInstruments, Avustralya</td><td>-</td><td></td></tr><tr><td>MgCl2&middot; 6H2O</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A100288</td><td></td></tr><tr><td>Çoklu miyograf sistemi&nbsp;</td><td>Danimarka Myo Technology, Aarhus, Danimarka</td><td>620 DK.</td><td></td></tr><tr><td>NaCl</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A100241</td><td></td></tr><tr><td>NaHCO3</td><td>Sangon Biotech Co., Ltd., Şanghay, Çin</td><td>A100865</td><td></td></tr><tr><td>Fenilefrin</td><td>Sigma-Aldrich, Merck, Darmstadt, Almanya</td><td>P6126</td><td></td></tr></tbody></table>

İnsan renal arteriyel dallarının Çok düzeyli mikrodiseksiyonu ve fonksiyonel-yapısal profillemesi

Multilevel Microdissection and Functional-Structural Profiling of Human Renal Arterial Branches

İnsan böbreğinde, interlobar arter, karşılık gelen damarı ile birlikte böbrek piramitleri arasında uzanır. Nispeten kalın bir vasküler duvar ve önemli yağ dokusu kaplaması ile karakterize edilen bu arter, özellikle dikkatli bir diseksiyon gerektirir. İzolasyon işlemi sırasında aşırı kuvvet uygulamamak için çevredeki yağ dokusu titizlikle çıkarılmalıdır (Şekil 1C).
Kavisli arter anatomik olarak kortikomedüller bileşkede konumlandırılmıştır ve böbrek piramitlerinin ...

İnsan Renal Arteriyel Dallarının Çok Düzeyli Mikrodiseksiyonu ve Fonksiyonel-Yapısal Profillemesi

Renal vascular dysfunction plays a critical role in the pathogenesis of multiple clinical conditions, including acute kidney injury, renal ischemia, and ...

multilevel-microdissection-functional-structural-profiling-human

Research

JoVE Journal

Medicine

Tıp

Vasküler disfonksiyon ve fizyolojik mekanizmaları katkı derecesi brakiyal arter akım aracılı dilatasyon, aort nabız dalga hızı ve vasküler endotelyal hücre proteini ifade ölçülerek, kronik böbrek hastalığı olan hastalarda değerlendirilebilir.

Kronik Böbrek Hastalarında Vasküler Fonksiyon Değerlendirilmesi

Zebra balığı yetişkin böbrek böbrek yenilenmesi ve hastalık çalışmalar için mükemmel bir sistemdir. Böyle bir araştırmanın önemli bir yönü nefron yapısı ve fonksiyonu değerlendirmesidir. Bu protokol nefron borucuk kompozisyonunun ve böbrek geri emiliminin değerlendirilmesi için uygulanabilecek çeşitli yöntemler anlatılmaktadır.

Yetişkin Zebra balığı Böbrek Nefron Kompozisyon ve Fonksiyon Analizi

Polikistik böbrek hastalığı için kemirgen modellerinin fenotipik bilgilerini elde etmek ve müdahaleleri izlemek için non-invaziv bir yol olarak ultra yüksek alan MRG'nin kullanımı açıklanmaktadır. Geleneksel histolojik yaklaşımla karşılaştırıldığında, MRG görüntüleri in vivo olarak elde edilebilir ve bu da uzunlamasına takibe izin verir.

Için Polikistik Böbrek Hastalığında Küçük Kemirgen modelleri Ultra yüksek Saha MRI Kullanımı<em&gt; In Vivo</em&gt; Fenotiplendirme ve İlaç İzleme

Bu protokol, fare embriyolarından metanefrik temelleri izole etmek ve kültürlemek için bir yöntem açıklamaktadır.

Fare Embriyonik Böbreklerinin Diseksiyonu ve Kültürü

Böbrek yapıları doku Mühendisliği zararlı etkileri diyaliz ve organ sıkıntısı için bir çözüm sağlar. Burada, bir protokol mikro tarif yalıtım kortiko-medüller segmentleri için fare böbrekler incelemek. Bu segmentler oluşturan böbrek organoids iskele-Alerjik Hücresel yapıları implante edilir.

Mikrodiseksiyon birincil böbrek doku parçaları ve roman iskele-Alerjik yapı teknolojisi ile birleşme

Renal arter darlığını başlatmak için poliüretan tüp kullanılarak modifiye edilmiş 2 böbrek 1 klips (2K1C) Goldblatt fare modeli geliştirildi ve renin ekspresyonunda ve böbrek hasarında artışa neden oldu. Burada, tekrarlanabilir ve tutarlı bir 2K1C fare modeli oluşturmak için manşetin renal arter üzerine hazırlanması ve yerleştirilmesi için ayrıntılı bir prosedür açıklıyoruz.

Renal Arter Darlığında Renin Regülasyonunun Modifiye İki Böbrek Bir Klipsli Fare Modeli

Glomerulus, proksimal tübül, kalın yükselen uzuv, toplama kanalı ve interstiyum dahil olmak üzere insan böbreğinin alt segmentlerinin lazer mikroseksiyonu için bir protokol açıklıyoruz. RNA daha sonra elde edilen bölmelerden izole edilir ve her alt segmentteki transkriptomik imzadaki değişiklikleri belirlemek için RNA dizilimi gerçekleştirilir.

İnsan Böbrek Dokusunda Bölgesel TranskriptomikLeri Ortaya Çıkarmak İçin Lazer Mikrodispeksiyon Uygulaması

Protokol, endotel hücrelerinin insan mezenterik arterinden izolasyonunu, kültürünü ve profillemesini tanımlar. Ek olarak, insan arterini mekansal transkriptomik için hazırlamak için bir yöntem sağlanmıştır. İzole hücreler üzerinde proteomik, transkriptomik ve fonksiyonel testler yapılabilir. Bu protokol, herhangi bir orta veya büyük boyutlu arter için yeniden kullanılabilir.

Transkriptom Düzeyinde İnsan Primer Mezenterik Arteriyel Endotel Hücrelerinin İzolasyonu ve Profillenmesi

Burada, uzun süreli üreter tıkanıklığının glomerüler dinamikler ve fonksiyon üzerindeki etkilerini ölçmek için Münih Wistar Fromter sıçanlarında yüzey glomerüllü 2-foton mikroskobu kullanan bir protokol sunuyoruz.

Kronik Tek Taraflı Üreter Tıkanıklığının Glomerüler Prosesler Üzerindeki Etkilerini Ölçmek için 2-Foton Mikroskobu Kullanılması

Burada, tüm böbrek, izole kortikal tübül ve medüller proteomların proteomik analizi için ayrıntılı bir protokol sunuyoruz. Çalışma ayrıca diyabetik bir fare modelinde ve diyabetik olmayan farelerde bölgesel proteomları karşılaştırır.

Farelerde Böbrek Hasarının Diyabetik Patogenezinde Tüm Böbrek, Medulla ve Kortikal Tübüllerin Karşılaştırmalı Proteomik Analizi

Keşfedin, insan renal arteriyel dallarının mikrodiseksiyonu ve tel miyografi ile fonksiyonel değerlendirmesini. Bu yöntem, vasküler disfonksiyon mekanizmalarını anlamaya ve hedefe yönelik tedaviler geliştirmeye yardımcı olur.

İnsan Renal Arteriyel Dallarının Çok Düzeyli Mikrodiseksiyonu ve Fonksiyonel-Yapısal Profillemesi

Daha fazla video keşfet