Name: Fusarium oxysporum f. sp. cubense Tropikal Irk 4ün Tespiti için Kombine Rekombinaz Polimeraz Amplifikasyonu CRISPR/Cas12a Testi (Video) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
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Description: Keşfedin, Fusarium oxysporum f. sp. cubense Tropikal Irk 4'ü tespit etmek için rekombinaz polimeraz amplifikasyonu ve CRISPR/Cas12a teknolojisini birleştiren bu hızlı, düşük maliyetli yöntemi.

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</ol>

<table><tbody><tr><td>EnGen Lba Cas12a (Cfp1) Proteini</td><td>New England Biolabs</td><td>M0653T</td><td></td></tr><tr><td>EnGen Lba Cas12a Diluent</td><td>New England Biolabs</td><td>B0653A</td><td></td></tr><tr><td>Eppendorf PCR Tüpleri</td><td>Merck</td><td>EP0030124332</td><td></td></tr><tr><td>Eppendorf Güvenli Kilitli Mikro Test Tüpleri</td><td>Merck</td><td>EP0030121708</td><td></td></tr><tr><td>Evrensel Çıkarma Torbaları</td><td>BIOREBA</td><td>430100</td><td></td></tr><tr><td>GeneRuler 100 bp DNA Merdiveni</td><td>Thermo Fischer Scientific</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>Genie Santrifüj Mini-6KO</td><td>OptiGene</td><td>OP-FUGE</td><td></td></tr><tr><td>Genie III</td><td>OptiGene</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>Genie Şerit Tutucu</td><td>OptiGene</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>Cin Şeritleri</td><td>OptiGene</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>HiScribe T7 Yüksek Verimli RNA Sentez Kiti</td><td>New England Biolabs</td><td>E2040S</td><td></td></tr><tr><td>NanoDrop ND-1000 UV/Vis Spektrofotometresi</td><td>Thermo Fischer Scientific</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>New England Biolabs</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>New England Biolabs</td><td>B6002S</td><td></td></tr><tr><td>Polivinilpirrolidon (PVP) tozu</td><td>Sigma-Aldrich</td><td>9003-39-8</td><td></td></tr><tr><td>RNA Temizliği ve Konsantretör-5 Kiti</td><td>Zymo Araştırması</td><td>R1013</td><td></td></tr><tr><td>RNA Yükleme Boya</td><td>Thermo Fischer Scientific</td><td>R0641</td><td></td></tr><tr><td>SmartBlue Mavi Işık Transilaydınatörü</td><td>Accuris Instruments</td><td>E4000-E</td><td></td></tr><tr><td>Sodyum hidroksit (NaOH) peletleri</td><td>Merck, EMPARTA</td><td>K277</td><td></td></tr><tr><td>Tris (hidroksilemetil) aminometan</td><td>Merck</td><td>77-86-1</td><td></td></tr><tr><td>TwistAmp Sıvı Temel Kit</td><td>TwistDx</td><td>TALQBAS01</td><td></td></tr></tbody></table>

fusarium oxysporum f. sp. cubense tropikal irk 4'ün tespiti için kombine rekombinaz polimeraz amplifikasyonu crispr/cas12a testi

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Fusarium oxysporum f. sp. cubense Tropikal Irk 4'ün Tespiti için Kombine Rekombinaz Polimeraz Amplifikasyonu CRISPR/Cas12a Testi

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

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Çevre

DNA, düzeneğini kullanarak çok CRISPR vektörleri CRISPR çok sayıda yapı yapım tek klonlama reaksiyonda paralel yapılabilir basit bir işlem vektörleri. Domates kıllı kökleri CRISPR vektörleri doğrulamak ve mutant malzemeleri üretmek için mükemmel bir model sistem.

Domates Tüylü Roots Üretimi ile yüksek verim CRISPR Vektör İnşaat ve DNA Değişiklikler Karakterizasyonu

Genetik Ustilago Maydis'in karalamak mantar işlemek için Biz sağlam bir gen değiştirme stratejisini açıklar. Bu protokol enfeksiyon fenotipleri araştırmak için silme mutantlarını üretmek açıklar. Bir floresan protein etiketi kodlayan bir sekansı ilave olarak, örneğin, arzu edilen herhangi bir şekilde, genleri değiştirme uzatılabilir.

Bitki patojeni olarak genetik manipülasyonu<em&gt; Ustilago maydis</em&gt; Mantar Biyoloji ve Bitki Mikrop Etkileşimleri Eğitim için

Burada, CRISPR-Cas9 ribonucleoproteins ve homoloji bağımlı onarım şablonları kullanarak C. elegans genom mühendisi bir yöntem mevcut.

Bir hızlı ve genomik noktası mutantlar C. elegans kullanarak CRISPR/Cas9 Ribonucleoproteins üreten Facile boru hattı

Bu protokolün amacı, C. elegans genomunun monte edilmiş ribonikleoprotein kompleksleri ve tarama için dpy-10 co-CRISPR işaretleyicisi kullanılarak sorunsuz CRISPR/Cas9 düzenlemesini sağlamaktır. Bu protokol, C. elegans'ta eklemeler, silmeler, gen değiştirmeleri ve kodon ikameleri de dahil olmak üzere çeşitli genetik değişiklikler yapmak için kullanılabilir.

CRISPR/Cas9 C. elegans rbm-3.2 Geninin dpy-10 Co-CRISPR Tarama İşaretçisi ve Monte Edilmiş Ribonikleoprotein Kompleksleri kullanılarak düzenlenmesi.

Bu çalışmada, Candidatus Liberibacter asiaticus için CRISPR-Cas12a ile kombine rekombinaz polimeraz amplifikasyonuna dayanan hızlı, hassas ve taşınabilir bir tespit yöntemi geliştirilmiştir.

CRISPR-Cas12a ile Kombine Rekombinaz Polimeraz Amplifikasyonu Kullanılarak Sahada Konuşlandırılabilir Candidatus Liberibacter asiaticus Tespiti

CRISPR-Cas sistemleri ve anti-CRISPR proteinleri, Saccharomyces cerevisiae'deki Boole kapılarının şemasına entegre edildi. Yeni küçük mantık devreleri iyi performans gösterdi ve hem dCas9 / dCas12a tabanlı transkripsiyon faktörlerinin hem de anti-CRISPR proteinlerinin özelliklerinin anlaşılmasını derinleştirdi.

CRISPR-Cas Sistemlerine ve Anti-CRISPR Proteinlerine Dayalı Gen Dijital Devreleri

DNA virüslerinin eser tespiti için rekombinaz polimeraz amplifikasyonunu bir CRISPR/Cas12a sistemi ile birleştiren bir protokol sunuyoruz ve hasta başı DNA virüsü tespiti için yapay zeka destekli bir sınıflandırma ile taşınabilir akıllı telefon mikroskobu oluşturuyoruz.

RPA-CRISPR/Cas12a-SPM ve Derin Öğrenmeye Dayalı DNA Virüs Tespit Sistemi

Burada, bulaşıcı hastalık patojenlerinin nükleik asit biyobelirteçlerinin tespiti için kullanılabilecek önceden karıştırılmış, liyofilize rekombinaz bazlı izotermal amplifikasyon ve Kümelenmiş Düzenli Aralıklı Kısa Palindromik Tekrarlar (CRISPR) tabanlı reaksiyonların adım adım hazırlanmasını sunuyoruz.

Önceden Karıştırılmış ve Dondurularak Kurutulmuş Reaktifler ile Bakım Noktası CRISPR Tabanlı Teşhis

Acinetobacter baumannii'nin hızlı ve hassas tespitini kolaylaştırmak için, A. baumannii enfeksiyonlarını tanımlamak için LbaCas12a endonükleaz ile birlikte Rekombinaz Polimeraz Amplifikasyonu (RPA) kullanan bir protokol sunuyoruz.

Rekombinaz polimeraz amplifikasyonu/Cas12a tabanlı bir sistem kullanılarak Acinetobacter baumannii enfeksiyonlarının hızlı ve spesifik tespiti

Burada, amplifikasyona hazır hücre lizzi, rekombinaz polimeraz amplifikasyonu ve CRISPR/Cas12a tahlilleri kullanarak Lotmaria passim ve Nosema ceranae gibi arı patojenlerinden DNA'yı tespit etmek için basit ve hızlı bir prosedürü açıklıyoruz.

RPA/CRISPR/Cas12a Sistemine Bağlı Alkalin Lizis Yoluyla Tripanosomatid Parazitlerin ve Nosema ceranae'nin Yerinde DNA Tespiti

Keşfedin, Fusarium oxysporum f. sp. cubense Tropikal Irk 4'ü tespit etmek için rekombinaz polimeraz amplifikasyonu ve CRISPR/Cas12a teknolojisini birleştiren bu hızlı, düşük maliyetli yöntemi.

Fusarium oxysporum f. sp. cubense Tropikal Irk 4'ün Tespiti için Kombine Rekombinaz Polimeraz Amplifikasyonu CRISPR/Cas12a Testi

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