Name: Tıbbi Bitkilerde Tropan Alkaloidlerin Nicelenmesi İçin Verimli ve Hızlı Bir HPLC-QQQ-MS yöntemi (Video) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: Keşfedin, HPLC-QQQ-MS yöntemiyle Anisodus tanguticus bitkisindeki tropan alkaloidlerinin (hiyosiyamin, anizodamin ve skopolamin) hassas ve hızlı nicelenmesini. Bu protokol, yüksek duyarlılık ve tekrarlanabilirlik sağlayarak tıbbi bitki kalite kontrolüne katkıda bulunur.

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<table><tbody><tr><td>Agilent MassHunter yazılımı</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 kolon</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 ters faz kolon, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>Anizodamin hidrobromür</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Formik Asit</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>dondurucu kurutucu</td><td>EYELA, Tokyo, Japonya</td><td>FDU-2110</td><td></td></tr><tr><td>Yüksek hızlı evrensel öğütücü</td><td>Taisite Instrument Co., Ltd., Tianjin, Çin</td><td>6010210100</td><td>FW-100,24,000 rpm, 100 g kapasite, 80 mesh</td></tr><tr><td>Hiosiyamin</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Metanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Mikro gözenekli Membran 0.22&mu;m</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 su sistemi</td><td>MilliporeSigma, Burlington, MA, ABD</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Skopolamin hidrobromür</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

tıbbi bitkilerde tropan alkaloidlerin nicelenmesi İçin verimli ve hızlı bir hplc-qqq-ms yöntemi

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

HPLC-QQQ-MS kromatografik ayrımı ve TA'ların tanımlanması Standart bir karışım ve A.tanguticus kök ekstraktının analizinden elde edilen tipik bir MRM kromatogramı, Şekil 3 ve Şekil 4'te sunulmaktadır. Optimize edilmiş HPLC koşullarında, üç hedef tropan alkaloidi olan hyoscyamin, anisodamin ve skopolamin 20 dakika içinde başarıyla ayrıldı ve geliştirilen yöntemin yüksek verimliliğini gösterdi. Skopola...

Tıbbi Bitkilerde Tropan Alkaloidlerin Nicelenmesi İçin Verimli ve Hızlı Bir HPLC-QQQ-MS yöntemi

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

an-efficient-rapid-hplc-qqq-ms-method-for-quantitation-tropane

Research

JoVE Journal

Chemistry

Kimya

Gerçek zamanlı yüksek çözünürlüklü kütle spektrometresi ve çok değişkenli istatistiksel analiz doğrudan analizi ile botanik malzemenin türü tanımlanması için bir yöntem sunulmuştur.

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Burada biz bir yüksek-den geçerek yöntemi ile kalite kontrol multisegment enjeksiyon-kılcal göre geliştirilmiş çözünürlük ve ilaç kötüye kullanımı ve bunların metabolitleri büyük paneller tespiti için sağlar kapsamlı uyuşturucu gözetimi için tarif Elektroforez-kütle spektrometresi.

Multisegment enjeksiyon-kılcal Elektroforez kütle spektrometresi kullanılarak yüksek üretilen iş ve kapsamlı uyuşturucu gözetim

Bitkilerde in vivo nükleozidler/nükleotidler nicelemesi için kesin ve tekrarlanabilir bir yöntem burada açıklanmıştır. Bu yöntem bir HPLC-MS/MS kullanmaktadır.

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Bu protokol, çay bahçelerindeki PA üreten yabani otlardan alınan çay örneklerinde pirolizidin alkaloidlerinin (PA'lar) kontaminasyonunu açıklamaktadır.

Çay Numunelerinde Pirrolizidin Alkaloid Kontaminasyonunun Kaynağı ve Yolu

Burada, hayvansal kaynaklı ilaçlarda trimetilamin (TMA) tayini için uygun bir headspace gaz kromatografisi-tandem dört kutuplu kütle spektrometrisi (HS-GC-MS/MS) yöntemi açıklanmaktadır. Protokol, numune ön işlemini, üst boşluk tedavisini, analiz koşullarını, metodolojik doğrulamayı ve hayvansal kaynaklı ilaçlarda TMA'nın belirlenmesini içerir.

Headspace Gaz Kromatografisi-Tandem Dört Kutuplu Kütle Spektrometrisi ile Hayvan Kaynaklı Tıpta Trimetilamin Tayini için Geliştirilmiş Bir Teknik

Hızlı kütle spektrometresi (MS)/kütle spektrometresi (MS) tabanlı tropan alkaloidlerinin açıklanması ve sınıflandırılması için hem tropan içeren numunelerin ön replikasyonu hem de izolasyon için yeni alkaloidlerin keşfi için yararlı bir yöntem sunuyoruz.

Doğal Ürün Araştırmalarında Sıvı Kromatografi Tandem Kütle Spektrometresinin Uygulamaları: Bir Vaka Çalışması Olarak Tropan Alkaloidleri

Bu protokol, yakın zamanda yayınlanan Komisyon Yönetmeliği (AB) 2023/915'e uygun olarak gıdalarda ergot alkaloidlerinin varlığını belirlemek için doğrulanmış bir sıvı kromatografisi-iyon hareketliliği-yüksek çözünürlüklü kütle spektrometresi yöntemi sunar.

Sıvı Kromatografisi Tuzaklı İyon Hareketlilik Spektrometresi-Uçuş Süresi Kütle Spektrometresi ile Gıda Matrislerinde Düzenlenmiş Ergot Alkaloidlerinin Tespiti

Bu protokol, reaktif saflaştırma, reaksiyon koşulları, Bioconjugate saflaştırma ve Bioconjugate karakterizasyonu dahil olmak üzere, bir maleimid proteini ihtiva eden bir sisteinin bioconjugation için gerekli olan önemli adımlar göstermektedir.

Protein Bioconjugatlar sentezi<em&gt; yoluyla</em&gt; Sistein-maleimid Kimya

Çok yüksek cam geçiş sıcaklığına sahip fonksiyonel polimerler oluşturmak için 5-norbornen-2-karboksilik asit ve 5-vinil-2-norbornenin katalitik yerleştirme polimerizasyonunu açıklıyoruz.

Fonksiyonel norbornenler Katalitik Yerleştirme Polimerizasyon için Basit ve Etkin Protokol

125-etiketli azid sentezi ve bir bakır içermeyen tıklama reaksiyonu kullanılarak dibenzocyclooctyne (DBCO) -grubu ile konjüge edilmiş 13-mil ölçekli altın nanopartiküllerinin radyo-etiketlenmesi için ayrıntılı bir işlem tarif edilmektedir.

Bir Kullanarak Altın Nanopartiküller Verimli Radyoaktif bir Optimize Protokolü<sup&gt; 125</sup&gt; I-etiketli Azid Protez Grubu

Keşfedin, HPLC-QQQ-MS yöntemiyle Anisodus tanguticus bitkisindeki tropan alkaloidlerinin (hiyosiyamin, anizodamin ve skopolamin) hassas ve hızlı nicelenmesini. Bu protokol, yüksek duyarlılık ve tekrarlanabilirlik sağlayarak tıbbi bitki kalite kontrolüne katkıda bulunur.

Tıbbi Bitkilerde Tropan Alkaloidlerin Nicelenmesi İçin Verimli ve Hızlı Bir HPLC-QQQ-MS yöntemi

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