ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Thomas, F. H., Vanderhyden, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Oocyte-granulosa+cell+interactions+during+mouse+follicular+development%3A+regulation+of+kit+ligand+expression+and+its+role+in+oocyte+growth%5BTitle%5D)+AND+%22Reprod+Biol+Endocrinol%22%5BJournal%5D)">Oocyte-granulosa cell interactions during mouse follicular development: regulation of kit ligand expression and its role in oocyte growth.</a> Reprod Biol Endocrinol. 4 (1), 19(2006).</li>
<li>Robker, R. L., Hennebold, J. D., Russell, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coordination+of+ovulation+and+oocyte+maturation%3A+a+good+egg+at+the+right+time%5BTitle%5D)+AND+%22Endocrinology%22%5BJournal%5D)">Coordination of ovulation and oocyte maturation: a good egg at the right time.</a> Endocrinology. 159 (9), 3209-3218 (2018).</li>
<li>Casey, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sex+hormones+and+health%5BTitle%5D)+AND+%22Nurs+N+Z%22%5BJournal%5D)">Sex hormones and health.</a> Nurs N Z. 23 (1), 24-28 (2017).</li>
<li>Kinnear, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+ovarian+stroma+as+a+new+frontier%5BTitle%5D)+AND+%22Reproduction%22%5BJournal%5D)">The ovarian stroma as a new frontier.</a> Reproduction. 160 (3), R25-R39 (2020).</li>
<li>Simon, L. E., Kumar, T. R., Duncan, F. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+vitro+ovarian+follicle+growth%3A+a+comprehensive+analysis+of+key+protocol+variables%5BTitle%5D)+AND+%22Biol+Reprod%22%5BJournal%5D)">In vitro ovarian follicle growth: a comprehensive analysis of key protocol variables.</a> Biol Reprod. 103 (3), 455-470 (2020).</li>
<li>Spector, I., Derech-Haim, S., Boustanai, I., Safrai, M., Meirow, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anti-M%C3%BCllerian+hormone+signaling+in+the+ovary+involves+stromal+fibroblasts%3A+a+study+in+humans+and+mice+provides+novel+insights+into+the+role+of+ovarian+stroma%5BTitle%5D)+AND+%22Hum+Reprod%22%5BJournal%5D)">Anti-Müllerian hormone signaling in the ovary involves stromal fibroblasts: a study in humans and mice provides novel insights into the role of ovarian stroma.</a> Hum Reprod. 39 (11), 2551-2564 (2024).</li>
<li>Kedem, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Activated+ovarian+endothelial+cells+promote+early+follicular+development+and+survival%5BTitle%5D)+AND+%22J+Ovarian+Res%22%5BJournal%5D)">Activated ovarian endothelial cells promote early follicular development and survival.</a> J Ovarian Res. 10 (1), 64(2017).</li>
<li>Converse, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multinucleated+giant+cells+are+hallmarks+of+ovarian+aging+with+unique+immune+and+degradation-associated+molecular+signatures%5BTitle%5D)+AND+%22PLoS+Biol%22%5BJournal%5D)">Multinucleated giant cells are hallmarks of ovarian aging with unique immune and degradation-associated molecular signatures.</a> PLoS Biol. 23 (6), e3003204(2025).</li>
<li>Bafor, E. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+of+single+cells+from+individual+mouse+ovaries+for+flow+cytometry+and+functional+analysis%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis.</a> STAR Protoc. 4 (4), 102710(2023).</li>
<li>Shepherd, T. G., Thériault, B. L., Campbell, E. J., Nachtigal, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Primary+culture+of+ovarian+surface+epithelial+cells+and+ascites-derived+ovarian+cancer+cells+from+patients%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients.</a> Nat Protoc. 1 (6), 2643-2649 (2006).</li>
<li>Beschta, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+rapid+and+robust+method+for+the+cryopreservation+of+human+granulosa+cells%5BTitle%5D)+AND+%22Histochem+Cell+Biol%22%5BJournal%5D)">A rapid and robust method for the cryopreservation of human granulosa cells.</a> Histochem Cell Biol. 156 (5), 509-517 (2021).</li>
<li>Tingen, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+macrophage+and+theca+cell-enriched+stromal+cell+population+influences+growth+and+survival+of+immature+murine+follicles+in+vitro%5BTitle%5D)+AND+%22Reproduction%22%5BJournal%5D)">A macrophage and theca cell-enriched stromal cell population influences growth and survival of immature murine follicles in vitro.</a> Reproduction. 141 (6), 809-820 (2011).</li>
<li>O'Mara, A. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+mirabegron+treatment+increases+human+brown+fat%2C+HDL+cholesterol%2C+and+insulin+sensitivity%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">Chronic mirabegron treatment increases human brown fat, HDL cholesterol, and insulin sensitivity.</a> J Clin Invest. 130 (5), 2209-2219 (2020).</li>
<li>Zink, N., Stock, A. -K., Vahid, A., Beste, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((On+the+neurophysiological+mechanisms+underlying+the+adaptability+to+varying+cognitive+control+demands%5BTitle%5D)+AND+%22Front+Hum+Neurosci%22%5BJournal%5D)">On the neurophysiological mechanisms underlying the adaptability to varying cognitive control demands.</a> Front Hum Neurosci. 12, 411(2018).</li>
<li>Zhao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoids%5BTitle%5D)+AND+%22Nat+Rev+Methods+Primers%22%5BJournal%5D)">Organoids.</a> Nat Rev Methods Primers. 2 (1), 94(2022).</li>
<li>Yang, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoids%3A+the+current+status+and+biomedical+applications%5BTitle%5D)+AND+%22MedComm%22%5BJournal%5D)">Organoids: the current status and biomedical applications.</a> MedComm. 4 (3), e274(2023).</li>
<li>Alzamil, L., Nikolakopoulou, K., Turco, M. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoid+systems+to+study+the+human+female+reproductive+tract+and+pregnancy%5BTitle%5D)+AND+%22Cell+Death+Differ%22%5BJournal%5D)">Organoid systems to study the human female reproductive tract and pregnancy.</a> Cell Death Differ. 28 (1), 35-51 (2021).</li>
<li>Pierson Smela, M. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Directed+differentiation+of+human+iPSCs+to+functional+ovarian+granulosa-like+cells+via+transcription+factor+overexpression%5BTitle%5D)+AND+%22eLife%22%5BJournal%5D)">Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression.</a> eLife. 12, e83291(2023).</li>
<li>Dipali, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Self-organizing+ovarian+somatic+organoids+preserve+cellular+heterogeneity+and+reveal+cellular+contributions+to+ovarian+aging%5BTitle%5D)+AND+%22Aging+Cell%22%5BJournal%5D)">Self-organizing ovarian somatic organoids preserve cellular heterogeneity and reveal cellular contributions to ovarian aging.</a> Aging Cell. 25 (1), e70333(2026).</li>
<li>Di Nisio, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Silk-ovarioids%3A+establishment+and+characterization+of+a+human+ovarian+primary+cell+3D-model+system%5BTitle%5D)+AND+%22Hum+Reprod+Open%22%5BJournal%5D)">Silk-ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system.</a> Hum Reprod Open. 2025 (3), hoaf042(2025).</li>
<li>Nikanfar, S., Leonel, E. C. R., Damdimopoulou, P., Flaws, J. A., Amorim, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effects+of+phthalate+exposure+on+human+ovarian+extracellular+matrix+composition%3A+insights+from+a+3D+spheroid+model%5BTitle%5D)+AND+%22Environ+Res%22%5BJournal%5D)">Effects of phthalate exposure on human ovarian extracellular matrix composition: insights from a 3D spheroid model.</a> Environ Res. 279, 121797(2025).</li>
<li>Briley, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reproductive+age-associated+fibrosis+in+the+stroma+of+the+mammalian+ovary%5BTitle%5D)+AND+%22Reproduction%22%5BJournal%5D)">Reproductive age-associated fibrosis in the stroma of the mammalian ovary.</a> Reproduction. 152 (3), 245-260 (2016).</li>
<li>Converse, A., Zaniker, E. J., Amargant, F., Duncan, F. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recapitulating+folliculogenesis+and+oogenesis+outside+the+body%3A+encapsulated+in+vitro+follicle+growth%5BTitle%5D)+AND+%22Biol+Reprod%22%5BJournal%5D)">Recapitulating folliculogenesis and oogenesis outside the body: encapsulated in vitro follicle growth.</a> Biol Reprod. 108 (1), 5-22 (2023).</li>
<li>Dean, M., Jin, V., Russo, A., Lantvit, D. D., Burdette, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exposure+of+the+extracellular+matrix+and+colonization+of+the+ovary+in+metastasis+of+fallopian-tube-derived+cancer%5BTitle%5D)+AND+%22Carcinogenesis%22%5BJournal%5D)">Exposure of the extracellular matrix and colonization of the ovary in metastasis of fallopian-tube-derived cancer.</a> Carcinogenesis. 40 (1), 41-51 (2019).</li>
<li>Charalambous, C., Webster, A., Schuh, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aneuploidy+in+mammalian+oocytes+and+the+impact+of+maternal+ageing%5BTitle%5D)+AND+%22Nat+Rev+Mol+Cell+Biol%22%5BJournal%5D)">Aneuploidy in mammalian oocytes and the impact of maternal ageing.</a> Nat Rev Mol Cell Biol. 24 (1), 27-44 (2023).</li>
<li>Rowley, J. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Low+molecular+weight+hyaluronan+induces+an+inflammatory+response+in+ovarian+stromal+cells+and+impairs+gamete+development+in+vitro%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Low molecular weight hyaluronan induces an inflammatory response in ovarian stromal cells and impairs gamete development in vitro.</a> Int J Mol Sci. 21 (3), 1036(2020).</li>
<li>Amargant, F., Vieira, C., Pritchard, M. T., Duncan, F. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Systemic+low-dose+anti-fibrotic+treatment+attenuates+ovarian+aging+in+the+mouse%5BTitle%5D)+AND+%22Geroscience%22%5BJournal%5D)">Systemic low-dose anti-fibrotic treatment attenuates ovarian aging in the mouse.</a> Geroscience. 47 (3), 3475-3495 (2024).</li>
</ol>

<table><tbody><tr><td>0.2 &mu;m, Polyethersulfone syringe filter</td><td>Corning</td><td>431229</td><td></td></tr><tr><td>1.5 mL microcentrifuge tubes</td><td>MIDSCI</td><td>PR-MCT15</td><td></td></tr><tr><td>100 mL glass beaker</td><td>Fisherbrand</td><td>FB100100</td><td></td></tr><tr><td>100 mm Petri dishes</td><td>Corning</td><td>351029</td><td></td></tr><tr><td>15 mL conical tubes</td><td>ThermoFisher Scientific</td><td>339651&nbsp;</td><td></td></tr><tr><td>24-well plates (TC-treated)</td><td>Corning</td><td>353047</td><td></td></tr><tr><td>28-gauge insulin needles</td><td>Exel International</td><td>26027</td><td></td></tr><tr><td>35 mm Petri dishes</td><td>Corning</td><td>351008</td><td></td></tr><tr><td>3&beta;-HSD antibody</td><td>Cosmo Bio</td><td>K0607</td><td>1:1000</td></tr><tr><td>4% Paraformaldehyde</td><td>Electron Microscopy Sciences</td><td>1574100</td><td></td></tr><tr><td>40 &micro;m cell strainer</td><td>Corning</td><td>431750</td><td></td></tr><tr><td>45 mm watch glass</td><td>Electron Microscopy Sciences</td><td>7054345</td><td></td></tr><tr><td>50 mL concial tubes</td><td>ThermoFisher Scientific</td><td>339652&nbsp;</td><td></td></tr><tr><td>60 mL sterile syringe</td><td>Air-Tite</td><td>ML60</td><td></td></tr><tr><td>60 mm TC-treated plates</td><td>Corning</td><td>353037</td><td></td></tr><tr><td>6-well plates (TC-treated)</td><td>Corning</td><td>353046</td><td></td></tr><tr><td>Agarose</td><td>Hoefer</td><td>GR140-500</td><td>Gel strength >1200 g/cm2; Gel Temperature 36 &deg;C</td></tr><tr><td>Autoclave sterilization pouch</td><td>Fisherbrand</td><td>01-812-55</td><td></td></tr><tr><td>Biotinylated Goat Anti-Mouse IgG</td><td>Vector Laboratories</td><td>BA-9200-1.5</td><td>1:200</td></tr><tr><td>Biotinylated Goat Anti-Rabbit IgG</td><td>Vector Laboratories</td><td>PK-6101</td><td>1:200</td></tr><tr><td>Biotinylated Goat Anti-Rat IgG</td><td>Vector Laboratories</td><td>BA-9400-1.5</td><td>1:100</td></tr><tr><td>Bluing reagent</td><td>Fisherbrand</td><td>23-245681</td><td></td></tr><tr><td>Centrifuge with swing bucket rotor for 50 mL conical tubes</td><td>ThermoFisher Scientific</td><td>Sorvall X1R Pro-MD</td><td></td></tr><tr><td>Class II biosafety cabinet</td><td>The Baker Company</td><td>SG403A</td><td></td></tr><tr><td>Cleaved Caspase-3 (CC3) antibody</td><td>Cell Signaling Technology</td><td>9579T</td><td>1:250</td></tr><tr><td>CO2 incubator</td><td>ThermoFisher Scientific</td><td>Heracell VIOS 160i CO2 Incubator</td><td></td></tr><tr><td>Collagenase, Type IV, powder</td><td>Gibco, Fisher Scientific</td><td>17-104-019</td><td></td></tr><tr><td>C-Series Mouse Cytokine Antibody Array 3 Kit</td><td>RayBiotech</td><td>AAM-CYT-3-8</td><td></td></tr><tr><td>Cytoseal XYL</td><td>Epredia</td><td>83124</td><td></td></tr><tr><td>Deoxyribonuclease I from bovine pancreas</td><td>Millipore Sigma</td><td>DN25-100MG</td><td>Dnase I</td></tr><tr><td>Dissecting microscissors</td><td>World Precision Instruments</td><td>WPI-14003</td><td></td></tr><tr><td>Dissecting scissors</td><td>World Precision Instruments</td><td>WPI-15922</td><td></td></tr><tr><td>Dissection microscope</td><td>Leica</td><td>MZ9.5, S9 series</td><td></td></tr><tr><td>DPBS, no calcium, no magnesium</td><td>Gibco, Fisher Scientific</td><td>14-190-250</td><td>pH 7.0&ndash;7.3</td></tr><tr><td>Eosin</td><td>Fisherbrand</td><td>23-314631</td><td></td></tr><tr><td>F4/80 antibody</td><td>Bio-Rad</td><td>MCA497G</td><td>1:50</td></tr><tr><td>Fetal Bovine Serum, certified, heat inactivated, United States</td><td>Gibco, ThermoFisher Scientific</td><td>10082147</td><td></td></tr><tr><td>Fine-tipped dissecting forceps</td><td>World Precision Instruments</td><td>WPI-14098</td><td></td></tr><tr><td>FOXL2 antibody</td><td>Abcam</td><td>ab246511</td><td>1:200</td></tr><tr><td>Graefe Forceps</td><td>Fine Science Tools</td><td>1105010</td><td></td></tr><tr><td>Hematoxylin</td><td>EK Industries</td><td>EKI 47971GL</td><td></td></tr><tr><td>Hemocytometer</td><td>MedOne</td><td>DHCN015</td><td></td></tr><tr><td>Imaging System</td><td>ThermoFisher Scientific</td><td>EVOS FL Auto</td><td></td></tr><tr><td>IntestiCult Organoid Growth Medium (Mouse)</td><td>STEMCELL Technologies</td><td>06005</td><td>Basal medium, Supplements 1 and 2</td></tr><tr><td>Laminar flow hood</td><td>IVFtech</td><td>IVFtech sterile workstation</td><td></td></tr><tr><td>Leibovitz's L-15 Medium</td><td>Gibco, Fisher Scientific</td><td>11415114</td><td></td></tr><tr><td>Microscope slides</td><td>Mercedes Scientific</td><td>MER 7255/90/WH/CC</td><td></td></tr><tr><td>MicroTissues 3D Petri Dish micro-mold spheroids</td><td>Millipore Sigma</td><td>Z764043-6EA</td><td>Silicone casts for agarose micromolds</td></tr><tr><td>Modified Davidson's Fixative</td><td>Electron Microscopy Sciences</td><td>6413350</td><td></td></tr><tr><td>OVCAR8-RFP cells</td><td></td><td></td><td>Gift from Joanna Burdette PhD, University of Illinois Chicago</td></tr><tr><td>Penicillin-Streptomycin</td><td>Millipore Sigma</td><td>P4333-100ML</td><td></td></tr><tr><td>Picrosirius Red staining solution</td><td>StatLab</td><td>STPSRPT</td><td></td></tr><tr><td>RPMI 1640 Medium (ATCC modification)</td><td>Gibco, Fisher Scientific</td><td>A1049101</td><td></td></tr><tr><td>Tabletop centrifuge</td><td>Eppendorf</td><td>Centrifuge 5430 R&nbsp;</td><td></td></tr><tr><td>Trypan blue</td><td>ThermoFisher Scientific</td><td>T10282</td><td></td></tr><tr><td>Trypsin-EDTA (0.05%), phenol red</td><td>Gibco, ThermoFisher Scientific</td><td>25300054</td><td></td></tr><tr><td>Vimentin antibody</td><td>Cell Signaling Technology</td><td>5741S</td><td>1:100</td></tr><tr><td>&alpha;MEM, GlutaMAX Supplement, no nucleosides</td><td>Gibco, Fisher Scientific</td><td>32561102</td><td></td></tr></tbody></table>

yumurtalık mikro ortamını İnceltmek: fare yumurtalık somatik hücrelerinin İzolasyon, 2d kültür ve organoid kültürü yöntemleri

Probing the Ovarian Microenvironment: Methods for Isolation, 2D Culture, and Organoid Culture of Mouse Ovarian Somatic Cells

Yumurtalık Mikro Ortamını İnceltmek: Fare Yumurtalık Somatik Hücrelerinin İzolasyon, 2D Kültür ve Organoid Kültürü Yöntemleri

The ovary consists of heterogeneous populations of somatic cells, both within the follicle and the surrounding stroma, which are critical to support ...

probing-ovarian-microenvironment-methods-for-isolation-2d-culture

Research

JoVE Journal

Developmental Biology

Gelişim Biyolojisi

Üç boyutlu bağlamında normal hücreleri Kültür, hücresel dönüşüm ve tümörogenez için gerekli erken olayları incelemek için alternatif bir yöntem temsil eder. Bu yöntem, normal yumurtalık ve yumurtalık kanseri oluşumu erken olayları incelemek için oviductal hücreleri büyümek için kullanılır.

Yumurtalıklar ve fallop borularında Üç Boyutlu Organ Kültür Aljinatta Hidrojeller

Bu protokol yenidoğan farelerde ve prepubertal farelerden alınan bireysel yumurtalık folikülleri gelen yumurtalıklarını kullanarak, fare yumurtalık dokusunun primer kültür / ko-kültür açıklar. kültür teknikleri yumurtalık üzerindeki dışsal ajanların etkisi soruşturma olanak veren yüksek fizyolojik bir şekilde gelişimini desteklemek ve yumurtalık foliküllerinin arasındaki etkileşimlerin.

Kültür ve Co-Kültür Mouse Yumurtalıklar ve Yumurtalık Foliküller

Burada, yumurtalık kanseri hücresi adezyonunu, invazyonunu ve proliferasyonunu araştırmak için bir araç olarak, birincil insan mezotelyal hücreleri ve hücre dışı matris ile katmanlanmış fibroblastlardan oluşan periton boşluğunun astarının üç boyutlu bir in vitro modelini oluşturmak için bir protokol sunuyoruz.

İnsan periton boşluğuna bir Organotipik Kültür Yumurtalık Kanseri Yaygınlaştırılması Erken Adımlar Modelleme

Burada, köklerinin genç farelerin kültürlü yumurtalık içinde seri kesit olmadan ölçmek için bir protokol mevcut. Ayirt bütün organ ve doku takas kullanarak, fiziksel kesit optik kesit ile değiştirilir. Bu yöntem örnek hazırlama ve görselleştirme organ bütünlüğü korur ve belirli hücre otomatik miktar kolaylaştırır.

Bütün Dağı ayirt ve kültürlü fare yumurtalıklarda folikül miktar

Yumurtalık dokusu kültürler folikül gelişim, yumurtlama ve folikül atrezi modelleri olarak kullanılabilir ve düzenleyici mekanizmaları dinamik yumurtalık işlemleri gösterir.

Mouse ovarium olayları görselleştirmek için yumurtalık doku kültürü

Sığır yumurtalık korteksinin in vitro kültürü ve beslenme Merdiven adım diyetinin yumurtalık mikroçevrişi üzerindeki etkisi sunulmuştur. Yumurtalık korteks parçaları yedi gün boyunca kültürlendi ve steroidler, sitokinler ve folikül evreleri değerlendirildi. Merdiven-Adım diyet tedavisi, kültürde folikül ilerlemesine neden olan steroidogenezi artırmıştı.

Sığır Yumurtalık Korteks Doku Kültürü

Bu protokol, farklı hastalık evrelerinden yumurtalık kanseri organoidlerinin oluşturulması için sistematik bir çerçeve sunar ve verimi artırmak ve sonraki uygulamalar için sağlam uzun vadeli genişleme sağlamak için hastaya özgü değişkenliğin zorluklarını ele alır. Doku işleme, tohumlama, ortam gereksinimlerini ayarlama ve immünofloresan boyama için ayrıntılı adımlar içerir.

Yumurtalık Kanseri Organoidlerinin Biyobankacılığı Stratejisi: Histolojik Alt Tipler ve Hastalık Evrelerinde Hastalar Arası Heterojenliğin Ele Alınması

Bu protokol, birincil insan yumurtalık yüzey epitel (hOSE) hücrelerinden üç boyutlu (3D) doku organoidlerinin oluşturulmasını açıklar. Protokol, yeni toplanan yumurtalıklardan hOSE'nin izolasyonunu, hOSE'nin hücresel genişlemesini, kriyoprezervasyon-çözme prosedürlerini ve organoid türevini içerir. İmmünofloresan, kantitatif analiz ve bir tarama platformu olarak sergileme yardımcı programı dahildir.

Doku Rejenerasyonunu İncelemek İçin Bir Platform Olarak İnsan Yumurtalık Yüzeyi Epitel Organoidleri

Bu protokol, in vitro ve in vivo olarak yüksek dereceli seröz yumurtalık karsinomu (HGSOC) fare modellerinin geliştirilmesini detaylandırır. Murin fallop tüpü organoidlerinin türetilmesinden, insan HGSOC genetiğini özetlemek için genetik modifikasyonlarından ve in vivo tümörlerin gelişimi için sinjeneik farelerin yumurtalık bursasına sokulmasından.

Yüksek Dereceli Seröz Over Karsinomu Modellemesi için Fallop Tüpü Epitel Hücrelerinden Fare Kaynaklı Organoid Hatların Geliştirilmesi

İn vitro gametogenezi tamamlayan yeni bir iskelesiz agaroz kalıbında yumurtalık foliküllerini kültürleme yöntemlerini açıklıyoruz.

İskelesiz Fare Yumurtalık Folikül Kültürü için Özelleştirilebilir Agaroz Kalıplarının Üretimi ve Kullanımı

Northwestern University, Chicago.  Fare yumurtalık somatik hücrelerinin stroma ile zenginleştirilmiş bir yumurtalık fraksiyonundan izole edilmesi ve iskelesiz bir yaklaşımla fare yumurtalık somatik organoidleri üretme yöntemlerini açıklıyoruz.

Yumurtalık Mikro Ortamını İnceltmek: Fare Yumurtalık Somatik Hücrelerinin İzolasyon, 2D Kültür ve Organoid Kültürü Yöntemleri

Daha fazla video keşfet