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JoVE Journal
Biology

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Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
 

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

Article DOI: 10.3791/1009
February 11th, 2009

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Summary February 11th, 2009

This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.

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