Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Purification and Visualization of Influenza A…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
Journal (Biology)
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
Microcontact Printing of Proteins for Cell Biology
Journal (Biology)
Microcontact Printing of Proteins for Cell Biology
 

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Article DOI: 10.3791/1105 • 09:36 min • February 9th, 2009
February 9th, 2009 •
1, 1, 1
1Department of Zoology, University of British Columbia - UBC

Chapters

Summary
Automatic Translation

The genome of the influenza A virus consists of eight separate complexes of RNA and proteins, termed viral ribonucleoprotein complexes (vRNPs). This paper describes the glycerol gradient purification and transmission electron microscopy visualization of influenza A vRNPs.

Tags

Purification Visualization Influenza A Viral Ribonucleoprotein Complexes VRNPs Viral Envelope Nuclear Import Replication Isolated VRNPs Procedure Disruption Detergents Glycerol Gradient Velocity Sedimentation SDS-PAGE Coomassie Blue Staining Peak Fractions Concentration Centrifugation Integrity Verification Transmission Electron Microscopy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter