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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
 

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Article DOI: 10.3791/1167
March 6th, 2009

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Summary March 6th, 2009

This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.

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