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Chapters
- 00:05Title
- 01:16Introduction
- 02:00Preparing the Strains for Gametogenesis
- 03:00Mating the Strains
- 05:20Preparing for Tetrad Dissection
- 07:56Dissecting the Tetrads
- 09:00Representative Results: Tetrad Analysis
- 09:20Conclusion
Mating and tetrad separation are required for genetic analysis in Chlamydomonas reinhardtii. Here we demonstrate standard methods for gametogenesis, mating, zygote germination and tetrad dissection. This protocol consists of an easy-to-follow series of steps that will make genetic approaches amenable to scientists who are less familiar with Chlamydomonas.
Tags
MatingTetrad SeparationChlamydomonas ReinhardtiiGenetic AnalysisCell BiologyGeneticsLife CycleGrowthManipulationGenomic ResourcesTransformabilityNucleusOrganellesVegetative DiploidsDominance AnalysisTetrad DissectionNuclear InheritanceOrganellar InheritanceAllelism TestEpistasy AnalysisMap-based CloningGametogenesisZygote Germination
