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Imaging Exocytose in Retinal bipolaren Zellen mit TIRF-Mikroskopie
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
DOI:

11:54 min

June 09, 2009

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Chapters

  • 00:05Title
  • 01:00Introduction
  • 01:29Dissection and Bipolar Cell Isolation
  • 05:11Bipolar Cell Loading and Wash Out
  • 08:26Patch Clamping and TIRFM Imaging
  • 10:38Representative TIRFM Imaging Results
  • 11:23Conclusion

Summary

Automatic Translation

In diesem Video zeigen wir, wie Label und visualisieren einzelne synaptische Vesikel Exozytose und Menschenhandel in Goldfische Netzhaut bipolaren Zellen mit insgesamt internen Reflexion Fluoreszenz (TIRF)-Mikroskopie.

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