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在子宫内和前体内的基因表达在小鼠视网膜神经节细胞的电穿孔
JoVE Journal
Biology
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JoVE Journal Biology
In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
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在子宫内和前体内的基因表达在小鼠视网膜神经节细胞的电穿孔

DOI:
10.3791/1333-v

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25:06 min

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September 24, 2009

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, ,
1Departments of Pathology and Cell Biology, and Neuroscience,Columbia University College of Physicians and Surgeons, 2Department of Ophthalmology,Columbia University College of Physicians and Surgeons

Chapters

  • 00:05Title
  • 01:07Introduction
  • 01:46In utero Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vivo
  • 08:40In utero Retinal Electroporation – Retrieval of Electroporated Retina at E18.5
  • 12:34Ex vivo Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vitro
  • 18:49Ex vivo Retinal Electroporation – Harvesting and Plating of GFP+ Retinal Explants (2 days post-electroporation)
  • 23:33Representative Results from in utero and ex vivo Retinal Electroporations
  • 25:23Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们目前的操纵基因表达的小鼠视网膜神经节细胞（RGC的）两个技术<em>在子宫内</em><em>体外</em>电。这些技术使之一，研究如何在基因表达的改变影响研资局的发展，轴突导向和功能特性。

Tags

In Utero ElectroporationEx Vivo ElectroporationGene ExpressionMouse Retinal Ganglion CellsRetinal DevelopmentAxon GuidanceRetinotopic OrganizationSynapse FormationTransgenic MiceGene ManipulationChick In Ovo ElectroporationNeonatal Mouse Pups ElectroporationAdult Rats ElectroporationEmbryonic Murine Retinae ElectroporationRGC DevelopmentAxon ProjectionsIn Vivo Electroporation

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