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Generering av Human CD40-aktiverade B-celler
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Human CD40-activated B cells
DOI:

13:27 min

October 16, 2009

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Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:46Preparation of Feeder Cells
  • 02:27Preparation of PBMC for CD40- Stimulation
  • 04:38Preparation of Feeder Cells
  • 08:33CD40- Stimulation
  • 09:50Subcultivation
  • 12:20Analysis of CD40- Activated B Cells
  • 12:55Conclusion

Summary

Automatic Translation

I denna video presenterar vi<em> Ex vivo</em> Generation och ökningen av mänsklig CD40-aktiverade B-celler (CD40-B) från perifera mononukleära blodceller (PBMC) genom stimulering med CD40 ligand och interleukin-4.

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