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Chapters
- 00:00Title
- 00:08Introduction
- 00:32Preprocessing of Optical Recordings
- 03:16Movement Detection
- 04:05Check Intervals Module
- 07:28Probing Heart Function with SOHA
- 11:28Conclusion
We have developed a Semi-automated Optical Heartbeat Analysis method (SOHA) for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts. We demonstrate the application of our methodology to the analysis of heart function in fruit fly and embryonic mouse hearts.
Tags
Semi-automatedOptical Heartbeat AnalysisSmall HeartsDrosophilaZebrafishEmbryonic Mouse HeartsSOHAMovement Detection AlgorithmCardiac MovementsContractile EventsRelaxation EventsPhysiologically Relevant ReadoutsSystolic IntervalsDiastolic IntervalsHeart RateHeartbeat ArrhythmicityHeart Diameter MeasurementsDiastoleSystoleFractional ShorteningFractional Area ChangesDigital File OutputSpreadsheet CompatibilityStatistical PowerUser Input RequiredHeart Function Analysis
