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JoVE Journal
Biology

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Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
 

Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

Article DOI: 10.3791/1519
September 22nd, 2009

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Summary September 22nd, 2009

Explants of the central region of rat lens epithelia differentiate synchronously when cultured in the presence of FGF-2. Immunofluorescence microscopy of such cultures can provides novel information about gene expression and signaling events associated with terminal differentiation.

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