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抗体分析的荧光素酶免疫沉淀系统(LIPS)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Please note that all translations are automatically generated.
Click here for the English version.
抗体分析的荧光素酶免疫沉淀系统(LIPS)
DOI:
10.3791/1549-v
•
12:19 min
•
October 07, 2009
•
Peter D. Burbelo
,
Kathryn H. Ching
,
Caitlin M. Klimavicz
,
Michael J. Iadarola
1
Neurobiology and Pain Therapeutics Section
,
National Institute of Dental and Craniofacial Research, National Institutes of Health
Chapters
00:00
Title
00:57
Introduction
01:29
Transfection of Plasmids for Production of Renilla-antigen Fusion Proteins
03:28
Harvesting Renilla-antigen Fusions
05:12
Preparing a Sera Master Plate
06:30
LIPS Assay
09:01
Measuring Luminescence and Data Analysis
10:49
LIPS Assay as a Method for Rapid and High Thoughput Sera Antigen Analysis
11:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
唇(荧光素酶免疫沉淀系统)技术方面进行了阐述。总体方法涉及表达嵌合基因编码融合海肾荧光素酶(中国人民大学)在哺乳动物细胞中的抗原。原油中国人民大学抗原的提取,然后准备,没有净化,量化抗体免疫检测。
Tags
Antibody Profiling
Luciferase Immunoprecipitation Systems (LIPS)
Comprehensively Understanding
Quantifying Antibody Profiles
Autoantigens
Infectious Agents
Disease Mechanisms
Markers
Substratify Disease
Clinical Features
Pathogenesis
Highly Quantitative Method
Patient Sera Antibody Responses
Pathogen Antigens
Infection
ELISAs
Humoral Serological Response Profiles
Universal Format
Dynamic Antibody Titer Ranges
Proteome
HIV
Informative
Single Antigen ELISA
Purified Antigens
Assay Optimization Steps
Article
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ADD TO PLAYLIST
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