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Chapters
- 00:00Title
- 00:35Introduction
- 01:50Enucleation of Murine Eyes and Dissection of Retinas
- 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
- 11:40Murine Organotypic Retinal Wholemount Culture
- 14:10Dissociation of Cultured Retinal Wholemounts
- 19:35Washing of Dissociated Cell Suspensions
- 24:49Conclusion
यह वीडियो लेख organotypic रेटिना wholemount संस्कृतियों और exogenously प्रेरित प्रभाव के विश्लेषण के लिए एक cytospin प्रक्रिया की स्थापना को दर्शाता है. Organotypic रेटिना wholemount संस्कृतियों की नकल<em> Vivo में</em> स्थिति और काफी प्रयोगात्मक अभिव्यक्ती लिए murine retinas की पहुंच की सुविधा, जबकि शास्त्रीय murine पशु मॉडल का नुकसान circumventing.
Tags
In Vivo-like Organotypic Murine Retinal Wholemount CultureTargeted Gene AblationsAnimal ModelsRetinal Gene FunctionTransgenic Mouse ModelsConditional Knockout Mouse ModelsPrimary Cell CultureRecombinant FactorsGene OverexpressionSiRNA-mediated Gene KnockdownControlled EnvironmentDissociated Cell CultureEndogenous SignalsExogenously Triggered EffectsPharmacological ManipulationOrganotypic Retinal Wholemount CulturesPhysiological In Vivo SituationNeuronal InteractionsConnections PreservedDissection PeculiaritiesEmbryonic EyesPostnatal EyesAdult Murine EyesNeuronal ApoptosisRetinal Cell Proliferation
