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Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
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Chapters
- 00:00Title
- 00:36Introduction
- 01:09Preparation of Hypothalamic Brain Slices
- 03:12Preparation of Glass Pipettes
- 03:57Current Clamp Recording
- 07:25Validation of Cell-Attached Recording Method
- 09:09Conclusion
Activity in neuronal systems often requires synchronous action potential discharges from neurons within a specific population. For example, pulses of gonadotropin-releasing hormone (GnRH) likely require coordinated activity between GnRH neurons. We present our methodological approach for reliably obtaining simultaneous electrophysiological recordings from the diffusely distributed GnRH neurons.
Tags
Gonadotropin-Releasing Hormone (GnRH)Green Fluorescent Protein (GFP)Hypothalamic SlicesPituitary ReleaseLuteinizing Hormone (LH)Follicle-stimulating Hormone (FSH)Reproductive FunctionHypothalamusMedian EminenceHypophysiotropic Portal SystemAnterior Pituitary GlandGonadotropinsSystemic CirculationEpisodic PulsesPeptide ContentReleasable PoolNeurosecretionSingle Unit ActivityMulti-unit RecordingsLH ReleaseGnRH SomataFibers
