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Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

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12:29 min

February 04, 2010

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12:29 min
February 04, 2010

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This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

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