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一个可逆的,非侵入性方法,气道阻力测量和支气管肺泡灌洗小鼠流体取样
JoVE Journal
Biology
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JoVE Journal
Biology
A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
Please note that all translations are automatically generated.
Click here for the English version.
一个可逆的,非侵入性方法,气道阻力测量和支气管肺泡灌洗小鼠流体取样
DOI:
10.3791/1720-v
•
09:58 min
•
April 13, 2010
•
Sumanth Polikepahad
,
Wade T. Barranco
,
Paul Porter
,
Bruce Anderson
,
Farrah Kheradmand
3
,
David B. Corry
3
1
Department of Medicine
,
Baylor College of Medicine (BCM)
,
2
Millenium Premier Group
,
3
Department of Immunology
,
Baylor College of Medicine (BCM)
Chapters
00:00
Title
00:49
Introduction
01:21
Intubation of Mice
03:39
Setting Up an Intravenous Line
05:06
Airway Resistance Measurements
06:22
Bronchoalveolar Lavage
07:44
Results of Airway Resistance Measurements
09:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
重复测量的啮齿类动物的呼吸生理学和呼吸道炎症细胞取样是可取的,但一般并不可行。在这里,我们描述了一种可重复的方法,口头插管的小鼠气道高反应和呼吸道炎症细胞取样的反复测量,允许。
Tags
Reversible
Non-invasive Method
Airway Resistance Measurements
Bronchoalveolar Lavage Fluid Sampling
Mice
Airway Hyperreactivity
Experimental Asthma Models
Repeated Procedures
AHR Measurements
BAL Fluid Sampling
Protocols
Intranasal Challenge
Allergen
Sham Treatment
Anesthetized
Orally Intubated
Mechanically Ventilated
Dose Response Curves
Respiratory System Resistance (RRS)
Acetylcholine (Ach) Chloride
Allergen-challenged Animals
Differential Enumeration Of Airway Cells
Repeated Measurements Of AHR And BAL Fluid Collection
Maximal Airway Hyperresponsiveness
Airway Eosinophilia
Allergen Challenge
Longitudinal Experimentation
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