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Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
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Biology
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JoVE Journal Biology
Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

DOI:
10.3791/1733-v

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11:26 min

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May 05, 2010

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, ,
1Department of Horticulture and Crop Science,The Ohio State University, 2Department of Plant Pathology,North Carolina State University

Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:34Gene Introduction Using Particle Bombardment
  • 04:51Automated Image Collection
  • 07:44Image Analysis
  • 09:14Representative Results of Gene Expression Imaging in Plant Tissues
  • 10:12Conclusion

Summary

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We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.

Tags

RoboticsDynamic Image AnalysisGene ExpressionGreen Fluorescent Protein (gfp)PromotersParticle BombardmentRobotic Image Collection SystemFluorescence Dissecting MicroscopeDigital CameraRobotics PlatformCulture DishesExpression ProfilesManual Image AlignmentImageReady SoftwareGFP Intensity MeasurementsImageJ SoftwareBackground Fluorescence

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