Harvesting Sperm and Artificial Insemination of Mice

Amanda R. Duselis; Paul B. Vrana

doi:10.3791/184

Harvesting Sperm and Artificial Insemination of Mice

JoVE Journal
Biology

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JoVE Journal Biology

Harvesting Sperm and Artificial Insemination of Mice

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10:00 min

April 28, 2007

DOI: 10.3791/184-v

Amanda R. Duselis¹, Paul B. Vrana¹

¹Dept. of Biological Chemistry,University of California, Irvine (UCI)

This protocol demonstrates methods for extracting sperm from the testes of males and then inseminating female mice. This procedure is useful when precise time is needed in developmental studies as well as transgenic work.

My name is Amanda Dli from the laboratory of Dr.Paul Verana at the University of California Irvine. Today I'm gonna be demonstrating the procedure of artificial insemination. This entails extracting the sperm from the male and then taking that sperm and and putting it into an ovulating female.

The reason you might wanna do this is because the male and female may not mate at the time of which you need or they may not mate at all. Again, this can also be used with frozen sperm if a mouse line is very important. This procedure is very important to see demonstrated because a lot of manuals and journals do not show the proper procedure for handling the mouse.

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