Embryo Timing

Embryo timing refers to the coordinated sequence and pace of events that guide an embryo from fertilization through successive stages of development. It depends on the timing of cell divisions, cell differentiation, movement, and communication, with molecular signals and gene regulation helping cells activate developmental programs in the appropriate order. Studying embryo timing helps biologists relate developmental stage to changes in form and function, compare normal and disrupted development, and investigate how environmental conditions or genetic variation affect embryogenesis. Accurate timing is also important in developmental biology, reproductive research, and the interpretation of embryonic experiments.

Embryo Timing - Related Videos

Research

JoVE Journal - Biology

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

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Cited by 65 •

2009

Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

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Cited by 3 •

2012

Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems.

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

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Cited by 7 •

2017

A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

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Cited by 4 •

2016

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

Visualizing Neural Crest Cell Migration in a Zebrafish Embryo Using Multi-Photon Time Lapse Imaging

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2025

Source: Williams, A. L., et al. Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos. J. Vis. Exp. (2017)This video showcases a multi-photon time-lapse imaging protocol to visualize the migration of neural crest cells in a zebrafish embryo in real-time with high resolution.

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