Journal
/
Neuroscience
/
Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

DOI:
10.3791/1850-v

•

09:51 min

•

March 02, 2010

•

, ,
1Biology,Johns Hopkins University

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:08Part 1: The EOG Recording Setup
  • 02:59Part 2: Preparing Electrodes
  • 05:11Part 3: Recording the EOG and Analyzing Data
  • 08:10Representative EOG Results
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

The electroolfactogram (EOG) recording is an informative, easy-to-conduct, and reliable way of assessing olfactory function at the level of the olfactory epithelium. This protocol describes a recording setup, mouse tissue preparation, data collection, and basic data analysis.

Tags

Mouse Olfactory Sensory NeuronsAir-phase Electroolfactogram RecordingOlfactionElectroolfactogram (EOG) RecordingOlfactory FunctionOlfactory EpitheliumGenetic Modification In MiceKnock-out MiceKnock-in MiceOlfactory Signal Transduction ComponentsRegulatory MechanismsOdorant DetectionCiliaIon ChannelsEOG Potential

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1850?article_type=v

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
check_url/1850?article_type=v

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
check_url/1850?article_type=v

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
check_url/1850?article_type=v

Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
check_url/1850?article_type=v

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis
check_url/1850?article_type=v

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
check_url/1850?article_type=v

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques
check_url/1850?article_type=v

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
check_url/1850?article_type=v

Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals
check_url/1850?article_type=v

Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

Read Article