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含量测定扩散的信号分子能够重新调整胚胎脊髓连合轴突
JoVE Journal
Biology
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JoVE Journal
Biology
Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
Please note that all translations are automatically generated.
Click here for the English version.
含量测定扩散的信号分子能够重新调整胚胎脊髓连合轴突
DOI:
10.3791/1853-v
•
09:28 min
•
March 08, 2010
•
Virginia M. Hazen*
1,2
,
Keith Phan*
1
,
Ken Yamauchi*
1,2
,
Samantha J. Butler
2
1
Department of Biological Sciences
,
University of Southern California
,
2
Neuroscience Graduate Program
,
University of Southern California
Chapters
00:00
Title
00:28
Introduction
01:14
Preparation of Aggregates of Transfected COS Cells Using Hanging Drops
02:20
Preparation of Dorsal Spinal Cord Explants
03:31
Priming Collagen
04:31
Dissection of Dorsal Spinal Cord Explants and Positioning with COS Cell Aggregates in Collagen Matrix
06:51
Visualization of Commissural Axons By Immunohistochemistry
08:03
Representative Images of Explants
08:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种检测评估的一种信号分子的能力,这里骨形态发生蛋白7(BMP7),调整连合轴突。一个胚胎脊髓背侧的外植体是培养毗邻COS细胞分泌候选人生长因子的总和。重新调整连合轴突生长外植体内免疫组织化学显示。
Tags
Axons
Signaling Molecules
Commissural Axons
Reorientation Assay
Extrinsic Signals
Intrinsic Signals
Axon Guidance
BMP Chemorepellent
Roof Plate
Netrin
Sonic Hedgehog (Shh)
Floor Plate
Spinal Cord
Embryonic Day (E) 11 Rats
Collagen Gels
Dorsal Spinal Explants
Tag1 Glycoprotein
Axonal Trajectory
COS Cell Aggregate
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