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JoVE Journal
Biology

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对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7
 
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对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7

Article DOI: 10.3791/1876-v 07:39 min May 17th, 2010
May 17th, 2010

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Summary

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Click here for the English version.

SNAP标签和剪辑标签蛋白标签制度,包括荧光染料分子,使特定的共价结合,一个活细胞中的兴趣的蛋白质。一旦克隆和表达,标记蛋白可用于许多下游应用的各种基材,而无需再次进行克隆。

Tags

细胞生物学,第39期,荧光,标签,成像,SNAP标签,标签,显微镜,阿格,表面上看,细胞内,融合
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