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Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
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Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Article DOI: 10.3791/1936-v • 16:48 min • March 15th, 2010
March 15th, 2010 •
1, 1
1Department of Physiology and Green Center for Systems Biology, University of Texas Southwestern Medical Center

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This protocol describes three Drosophila preparations: 1) adult brain dissection, 2) adult retina dissection and 3) developing eye disc- brain complexes dissection. Emphasis is laid on special preparation techniques and conditions for live imaging, although all preparations can be used for fixed tissue immunohistochemistry.

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Drosophila Brain Retina Live Imaging Neuronal Development Function Degeneration Eye Disc-brain Complex Pupae Adult Flies Live Culture Fixed Tissue Immunohistochemistry Confocal Live Imaging Perfusion Chamber Time Scales
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