Quantification of &gamma;H2AX Foci in Response to Ionising Radiation

Li-Jeen Mah; Raja S. Vasireddy; Michelle M. Tang; George T. Georgiadis; Assam El-Osta; Tom C. Karagiannis

doi:10.3791/1957

Journal

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Biology

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Quantification of γH2AX Foci in Response to Ionising Radiation

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

Quantification of γH2AX Foci in Response to Ionising Radiation

20,317 Views

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06:53 min

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April 06, 2010

DOI:

10.3791/1957-v

DOI:

10.3791/1957-v

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06:53 min

April 06, 2010

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18 Views

Li-Jeen Mah^1,2, Raja S. Vasireddy^1,2,3, Michelle M. Tang^1,3, George T. Georgiadis¹, Assam El-Osta^2,3, Tom C. Karagiannis^1,2

¹Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, ²Department of Pathology,The University of Melbourne, ³Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct

Summary

Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.