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Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

JoVE Journal
Biology

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JoVE Journal Biology

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

DOI:

•

07:32 min

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June 18, 2010

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John Zaborske, Tao Pan

¹Department of Biochemistry and Molecular Biology,University of Chicago

Chapters

00:00Title
00:45Introductions
01:06Isolation of Total Charged tRNA
02:37Cy3/Cy5 Labeling of tRNA
05:15Hybridization and Analysis of the Microarray
06:33Representative Results of Isolation and Analysis of tRNA
07:09Conclusion

Summary

Automatic Translation

We describe a method for microarray analysis to determine relative aminoacylation levels of all tRNAs from S. cerivisiae.

Tags

Genome-wide Analysis Aminoacylation Charging Levels TRNA Microarray Translational Regulation Stress Response Stringent Response Gcn2 Stress Response Pathway Dynamic Charging Patterns Genomic Scale Environmental Variations Relative Charging Levels S. Cerevisiae Yeast Protocol E. Coli Human Cell Cultures

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