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Large-Scale Screens of Metagenomic Libraries

JoVE Journal
Biology

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JoVE Journal Biology

Large-Scale Screens of Metagenomic Libraries

Large-Scale Screens of Metagenomic Libraries

DOI:

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16:05 min

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May 28, 2007

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Vinh D. Pham, Tsultrim Palden, Edward F. DeLong

¹Division of Biological Engineering, Department of Civil and Environmental Engineering,MIT – Massachusetts Institute of Technology

Chapters

00:02Membrane filters
00:48Large-scale inoculation of bacterial colonies on membrane
03:00Fixing colony DNA on membrane
05:55Hybridization
08:10Washing membranes after hybridization
10:49Detection of the signal

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