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Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
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Chapters
- 00:00Title
- 01:03Introduction
- 01:39Preparation of Bacterial ( N. meningitidis) Suspension
- 03:09Seeding and Infection of Target Cells
- 04:29Immuno-staining
- 06:21Confocal Laser Scanning Microscopy
- 08:56Quantitative Colocalization Analysis
- 09:55Intracellular Localization of Opc-expressing N. meningitidis and Alpha-actinin
- 12:23Conclusion
Neisseria meningitidis (Nm), a gram negative human-specific respiratory pathogen, can bind to human α-actinin. Here we present a protocol for visualisation of colocalisation of the bacterium with intracellular α-actinin after bacterial entry into human brain microvascular endothelial cells (HBMECs).
