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Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

DOI:

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09:08 min

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December 11, 2010

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Tadeusz J. Kaczynski, Shermali Gunawardena

¹Department of Biological Sciences,SUNY-University at Buffalo

Chapters

00:05Title
00:50Introduction
01:08First Preparation of Reagents and Collection of Embryos
02:51Removal of the Chorion Vitelline Membranes and Fixation
05:21Antibody Staining, Mounting, and Visualization
08:07Representative Images
08:45Conclusion

Summary

Automatic Translation

We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis.

Tags

Visualization Embryonic Nervous System Whole-mount Drosophila Embryos Cellular Development Molecular Basis Neuronal Development Antibodies Peripheral Nervous System Central Nervous System Aberrations Neuronal Proteins Developmental Stages Fixation Fluorescently Labeled Secondary Antibodies CNS Germ Band Commissure Pattern PNS Development

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