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Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila
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Neuroscience
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JoVE Journal Neuroscience
Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

DOI:
10.3791/2157-v

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18:08 min

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September 28, 2010

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, , , ,
1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Current Address: Department of Entomology, College of Agricultural and Environmental Sciences,University of California, Davis, 3Department of Molecular Biology and Biochemistry,Rutgers University

Chapters

  • 00:00Title
  • 01:20Setting Up the Locomotor Activity Monitoring System
  • 03:50Preparation of Experimental Animals
  • 05:08Preparation of Activity Tubes
  • 10:03Loading Flies into Activity Tubes and Locomotor Activity Monitoring System
  • 11:36Experimental Design to Record Data for Determination of Circadian Periodicity and Amplitude
  • 13:54Representative Circadian Sleep/Rest Parameters
  • 17:47Conclusion

Summary

Automatic Translation

Automatic Translation

We describe procedures for recording daily locomotor activity rhythms of Drosophila and subsequent data analysis. Locomotor activity rhythms are a reliable behavioral output of animal circadian clocks and are used as the standard readout of clock function when studying circadian mutants or examining how the environment regulates the circadian system.

Tags

Assaying Locomotor ActivityCircadian RhythmsSleep ParametersDrosophilaEndogenous Circadian PacemakersClock FunctionGenetic ScreensCellular And Biochemical BasesLocomotor MovementMonitoring SystemInfrared LightDaily Cycles Of Activity And InactivityCommercially Available Monitoring DevicesSoftware ProgramsData Analysis

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