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Eletroforese em Gel de Poliacrilamida azul Nativo (BN-PAGE) para Análise de Complexos Multiproteicos de Lisados ​​Celular
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Eletroforese em Gel de Poliacrilamida azul Nativo (BN-PAGE) para Análise de Complexos Multiproteicos de Lisados ​​Celular

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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Neste vídeo, descrevemos a caracterização de complexos multiprotein (PPM) por eletroforese em gel de poliacrilamida nativo azul (BN-PAGE). Em uma primeira dimensão, dialisadas lisados ​​celulares são separados por BN-PAGE para identificar PPM individual. Em uma segunda dimensão SDS-PAGE, PPM de interesse são subdivididos para analisar os seus constituintes por immunoblotting.

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Bioquímica Edição 48 BN-PAGE eletroforese em gel 2D lisado celular diálise complexo de proteínas complexo multiprotein a interação de proteínas
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